Rabbit anti-Human APCS Polyclonal Antibody | anti-APCS antibody

APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203))

Cat. Num. AAA647035

• Gene Names: APCS; SAP; PTX2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: APCS; Polyclonal Antibody; CT (APCS; PTX2; Serum amyloid P-component; 9.5S alpha-1-glycoprotein; Serum amyloid P-component(1-203)); Anti -APCS; anti-APCS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-APCS antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: APCS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the C-terminal region of human APCS.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS647035. This demonstrates that MBS647035 detected the APCS protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS647035 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-APCS antibody

The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Product Categories/Family for anti-APCS antibody

Antibodies; Abs to Amyloid

NCBI and Uniprot Product Information

• NCBI GI #: 47496573
• NCBI GeneID: 325
• UniProt Accession #: P02743
• Molecular Weight: 25,387 Da
• NCBI Official Full Name: APCS
• NCBI Official Synonym Full Names: amyloid P component, serum
• NCBI Official Symbol: APCS
• NCBI Official Synonym Symbols: SAP; PTX2
• NCBI Protein Information: serum amyloid P-component; pentaxin-related; 9.5S alpha-1-glycoprotein
• UniProt Protein Name: Serum amyloid P-component
• UniProt Gene Name: APCS
• UniProt Synonym Gene Names: PTX2; SAP
• UniProt Entry Name: SAMP_HUMAN

NCBI Description

The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]

Uniprot Description

Function: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.

Cofactor: Binds 2 calcium ions per subunit.

Subunit structure: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.

Subcellular location: Secreted.

Tissue specificity: Found in serum and urine. Ref.10

Post-translational modification: N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (Ref.10). Ref.9

Involvement in disease: SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.

Sequence similarities: Belongs to the pentaxin family.Contains 1 pentaxin domain.

Mass spectrometry: Molecular mass is 25462.5±1.1 Da from positions 20 - 223. Determined by ESI. Ref.9Molecular mass is 25463±3 Da from positions 20 - 223. Determined by MALDI. Ref.10Molecular mass is 1285.78 Da from positions 213 - 223. Determined by MALDI. Ref.10Molecular mass is 1186.71 Da from positions 213 - 222. Determined by MALDI. Ref.10