ABCB10 sirna

ABCB10 siRNA (Mouse)

Cat. Num. AAA8228481

• Gene Names: Abcb10; Abc-me; Abcb12
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABCB10; ABCB10 siRNA (Mouse); ATP-binding cassette sub-family B member 10 mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein; ABCB10 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ABCB10 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 715

• Applicable Applications for ABCB10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ABCB10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABCB10 sirna

siRNA to inhibit ABCB10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9506367
• NCBI GeneID: 56199
• NCBI Accession #: NP_062425.1
• NCBI GenBank Nucleotide #: NM_019552.2
• UniProt Accession #: Q9JI39; Q542P7; Q9D0C7
• Molecular Weight: 77,188 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 10, mitochondrial
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 10
• NCBI Official Symbol: Abcb10
• NCBI Official Synonym Symbols: Abc-me; Abcb12
• NCBI Protein Information: ATP-binding cassette sub-family B member 10, mitochondrial
• UniProt Protein Name: ATP-binding cassette sub-family B member 10, mitochondrial
• Protein Family: ABC transporter B family
• UniProt Gene Name: Abcb10
• UniProt Synonym Gene Names: ABC-me protein; ABC transporter 10 protein
• UniProt Entry Name: ABCBA_MOUSE

NCBI Description

This gene encodes a member of the ATP-binding cassette superfamily of transporters. ATP-binding cassette proteins transport various molecules across extra- and intra-cellular membranes. The encoded protein is localized to the mitochondrial inner membrane where it interacts with and stabilizes mitoferrin-1, and is important for heme biosynthesis. Additional evidence suggests the encoded protein is involved in oxidative stress protection and erythropoisesis. [provided by RefSeq, May 2013]

Uniprot Description

ABCB10: May mediate critical mitochondrial transport functions related to heme biosynthesis. Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.

Protein type: Mitochondrial; Transporter; Transporter, ABC family; Hydrolase; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: protein binding; protein homodimerization activity; transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATPase activity; nucleotide binding; ATP binding

Biological Process: transport; transmembrane transport