ZNF274 sirna

ZNF274 siRNA (Human)

Cat. Num. AAA8228010

• Gene Names: ZNF274; ZF2; HFB101; ZSCAN51; ZKSCAN19
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZNF274; ZNF274 siRNA (Human); ZKSCAN19; Neurotrophin receptor-interacting factor homolog; Zinc finger protein 274; Zinc finger protein HFB101; Zinc finger protein with KRAB and SCAN domains 19; Zinc finger protein zfp2; Zf2; ZNF274 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZNF274 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 611

• Applicable Applications for ZNF274 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZNF274 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZNF274 sirna

siRNA to inhibit ZNF274 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 523567538
• NCBI GeneID: 10782
• NCBI Accession #: NP_001265663.1
• NCBI GenBank Nucleotide #: NM_001278734.1
• UniProt Accession #: Q96GC6; Q53XU4; Q6MZG1; Q8WY37; Q8WY38; Q92969; Q9UII0; Q9UII1
• Molecular Weight: 42,448 Da
• NCBI Official Full Name: neurotrophin receptor-interacting factor homolog isoform d
• NCBI Official Synonym Full Names: zinc finger protein 274
• NCBI Official Symbol: ZNF274
• NCBI Official Synonym Symbols: ZF2; HFB101; ZSCAN51; ZKSCAN19
• NCBI Protein Information: neurotrophin receptor-interacting factor homolog
• UniProt Protein Name: Neurotrophin receptor-interacting factor homolog
• Protein Family: Neurotrophin receptor-interacting factor
• UniProt Gene Name: ZNF274
• UniProt Synonym Gene Names: ZKSCAN19; Zf2
• UniProt Entry Name: ZN274_HUMAN

NCBI Description

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008]

Uniprot Description

ZNF274: Probable transcription repressor. Specifically binds to the 3'-end of zinc-finger coding genes and recruiting chromatin- modifying proteins such as SETDB1 and TRIM28/KAP1, leading to transcription repression. Belongs to the krueppel C2H2-type zinc-finger protein family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein; Nucleolus; DNA-binding

Chromosomal Location of Human Ortholog: 19qter

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription corepressor activity; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent