WDPCP sirna

WDPCP siRNA (Human)

Cat. Num. AAA8226575

• Gene Names: WDPCP; FRTZ; BBS15; FRITZ; CHDTHP; C2orf86
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WDPCP; WDPCP siRNA (Human); BBS15; C2orf86; FRITZ; WD repeat-containing and planar cell polarity effector protein fritz homolog; hFRTZ; Bardet-Biedl syndrome 15 protein; WD repeat-containing and planar cell polarity effector protein; WDPCP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WDPCP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 587

• Applicable Applications for WDPCP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WDPCP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WDPCP sirna

siRNA to inhibit WDPCP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 111548654
• NCBI GeneID: 51057
• NCBI Accession #: NP_001036157.1
• NCBI GenBank Nucleotide #: NM_001042692.2
• UniProt Accession #: O95876; Q53RW4; Q7Z2Z3
• Molecular Weight: 66,655 Da
• NCBI Official Full Name: WD repeat-containing and planar cell polarity effector protein fritz homolog isoform 1
• NCBI Official Synonym Full Names: WD repeat containing planar cell polarity effector
• NCBI Official Symbol: WDPCP
• NCBI Official Synonym Symbols: FRTZ; BBS15; FRITZ; CHDTHP; C2orf86
• NCBI Protein Information: WD repeat-containing and planar cell polarity effector protein fritz homolog
• UniProt Protein Name: WD repeat-containing and planar cell polarity effector protein fritz homolog
• Protein Family: WD repeat-containing and planar cell polarity effector protein
• UniProt Gene Name: WDPCP
• UniProt Synonym Gene Names: BBS15; C2orf86; FRITZ; hFRTZ
• UniProt Entry Name: FRITZ_HUMAN

NCBI Description

This gene encodes a cytoplasmic WD40 repeat protein. A similar gene in frogs encodes a planar cell polarity protein that plays a critical role in collective cell movement and ciliogenesis by mediating septin localization. Mutations in this gene are associated with Bardet-Biedl syndrome 15 and may also play a role in Meckel-Gruber syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

LOC51057: Probable effector of the planar cell polarity signaling pathway which regulates the septin cytoskeleton in both ciliogenesis and collective cell movements. Defects in WDPCP are the cause of Bardet-Biedl syndrome type 15 (BBS15). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Mutations in WDPCP may act as modifiers of the phenotypic expression of Bardet-Biedl syndrome and Meckel syndrome by interacting in trans with primary BBS and MKS loci. Belongs to the WD repeat fritz family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: cytoskeleton; apical plasma membrane; plasma membrane; cell cortex; axoneme

Biological Process: smoothened signaling pathway; establishment of protein localization; regulation of protein localization; camera-type eye development; septin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; auditory receptor cell morphogenesis; cilium biogenesis; palate development; embryonic digit morphogenesis; kidney development; shape changes of embryonic cells

Disease: Congenital Heart Defects, Hamartomas Of Tongue, And Polysyndactyly; Bardet-biedl Syndrome 15