Human pPLA2 ELISA Kit | pPLA2 elisa kit

Human pPLA2 (Phospholipase A2, Pancreas) ELISA Kit

Cat. Num. AAA2507688

• Gene Names: PLA2G1B; PLA2; PLA2A; PPLA2
• Reactivity: Human
• Synonyms: pPLA2; Human pPLA2 (Phospholipase A2; Pancreas) ELISA Kit; pPLA2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human pPLA2. No significant cross-reactivity or interference between Human pPLA2 and analogues was observed.
• Sequence Length: 148

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human pPLA2 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for pPLA2 elisa kit

Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with pPLA2. During the reaction, pPLA2 in the sample or standard competes with a fixed amount of pPLA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to pPLA2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of pPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505847
• NCBI GeneID: 5319
• NCBI Accession #: NP_000919.1
• NCBI GenBank Nucleotide #: NM_000928.2
• UniProt Accession #: P04054; Q3KPI1; B2R4H5
• Molecular Weight: 16,360 Da
• NCBI Official Full Name: phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group IB (pancreas)
• NCBI Official Symbol: PLA2G1B
• NCBI Official Synonym Symbols: PLA2; PLA2A; PPLA2
• NCBI Protein Information: phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B
• UniProt Protein Name: Phospholipase A2
• UniProt Gene Name: PLA2G1B
• UniProt Synonym Gene Names: PLA2; PLA2A; PPLA2
• UniProt Entry Name: PA21B_HUMAN

NCBI Description

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

Uniprot Description

PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - linoleic acid; Secreted, signal peptide; Lipid Metabolism - alpha-linolenic acid; DNA replication; Secreted; Lipid Metabolism - arachidonic acid; Phospholipase; EC 3.1.1.4; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space; cell surface; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; bile acid binding; calcium ion binding; receptor binding

Biological Process: positive regulation of immune response; activation of MAPK activity; neutrophil mediated immunity; activation of phospholipase A2; glucose transport; signal transduction; activation of NF-kappaB transcription factor; phosphatidylcholine metabolic process; positive regulation of fibroblast proliferation; phospholipid metabolic process; fatty acid biosynthetic process; multicellular organismal lipid catabolic process; neutrophil chemotaxis; glycerophospholipid biosynthetic process; actin filament organization; arachidonic acid secretion; leukotriene biosynthetic process; defense response to Gram-positive bacterium; cellular response to insulin stimulus; innate immune response in mucosa; positive regulation of protein secretion; antibacterial humoral response; phosphatidic acid biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA replication; interleukin-8 production