VTI1A sirna

VTI1A siRNA (Human)

Cat. Num. AAA8209675

• Gene Names: VTI1A; MMDS3; MVti1; VTI1RP2; Vti1-rp2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VTI1A; VTI1A siRNA (Human); Vesicle transport through interaction with t-SNAREs homolog 1A; Vesicle transport v-SNARE protein Vti1-like 2; Vti1-rp2; VTI1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: VTI1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 217

• Applicable Applications for VTI1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human VTI1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VTI1A sirna

siRNA to inhibit VTI1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 113374156
• NCBI GeneID: 143187
• NCBI Accession #: NP_660207.2
• NCBI GenBank Nucleotide #: NM_145206.2
• UniProt Accession #: Q96AJ9; Q5W0D7; A2A307; B4E137
• Molecular Weight: 23,435 Da
• NCBI Official Full Name: vesicle transport through interaction with t-SNAREs homolog 1A
• NCBI Official Synonym Full Names: vesicle transport through interaction with t-SNAREs 1A
• NCBI Official Symbol: VTI1A
• NCBI Official Synonym Symbols: MMDS3; MVti1; VTI1RP2; Vti1-rp2
• NCBI Protein Information: vesicle transport through interaction with t-SNAREs homolog 1A
• UniProt Protein Name: Vesicle transport through interaction with t-SNAREs homolog 1A
• Protein Family: Vesicle transport through interaction with t-SNAREs
• UniProt Gene Name: VTI1A
• UniProt Entry Name: VTI1A_HUMAN

Uniprot Description

VTI1A: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non- conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence. Belongs to the VTI1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: SNARE complex; Golgi membrane; Golgi apparatus; synaptic vesicle; endoplasmic reticulum membrane; Golgi stack; clathrin-coated vesicle; cell soma; perinuclear region of cytoplasm; late endosome membrane; integral to membrane; endosome

Molecular Function: SNAP receptor activity; SNARE binding

Biological Process: intra-Golgi vesicle-mediated transport; ER to Golgi vesicle-mediated transport; voluntary musculoskeletal movement; Golgi to vacuole transport; protein targeting to vacuole; vesicle fusion with Golgi apparatus; retrograde transport, endosome to Golgi

Disease: Multiple Mitochondrial Dysfunctions Syndrome 3