UGT2B15 sirna

UGT2B15 siRNA (Human)

Cat. Num. AAA8213637

• Gene Names: UGT2B15; HLUG4; UGT2B8; UDPGTH3; UDPGT 2B8; UDPGT2B15
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UGT2B15; UGT2B15 siRNA (Human); UGT2B8; UDP-glucuronosyltransferase 2B15; UDPGT 2B15; HLUG4; UDP-glucuronosyltransferase 2B8; UDPGT 2B8; UDPGTh-3; UGT2B15 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UGT2B15 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 530

• Applicable Applications for UGT2B15 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UGT2B15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UGT2B15 sirna

siRNA to inhibit UGT2B15 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116517299
• NCBI GeneID: 7366
• NCBI Accession #: NP_001067.2
• NCBI GenBank Nucleotide #: NM_001076.3
• UniProt Accession #: P54855; P23765; Q9UK63; A6NDX0; A6NNJ4; A8K054; AAB28536
• Molecular Weight: 61,036 Da
• NCBI Official Full Name: UDP-glucuronosyltransferase 2B15
• NCBI Official Synonym Full Names: UDP glucuronosyltransferase 2 family, polypeptide B15
• NCBI Official Symbol: UGT2B15
• NCBI Official Synonym Symbols: HLUG4; UGT2B8; UDPGTH3; UDPGT 2B8; UDPGT2B15
• NCBI Protein Information: UDP-glucuronosyltransferase 2B15
• UniProt Protein Name: UDP-glucuronosyltransferase 2B15
• Protein Family: UDP-glucuronosyltransferase
• UniProt Gene Name: UGT2B15
• UniProt Synonym Gene Names: UGT2B8; UDPGT 2B15; UDPGT 2B8
• UniProt Entry Name: UDB15_HUMAN

NCBI Description

This gene encodes a member of the UDP-glycosyltransferase (UDPGT) family. The UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This protein displays activity towards several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens. [provided by RefSeq, Oct 2011]

Uniprot Description

UGT2B15: UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme displays activity toward several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens. Belongs to the UDP-glycosyltransferase family.

Protein type: Carbohydrate Metabolism - ascorbate and aldarate; Lipid Metabolism - androgen and estrogen; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Carbohydrate Metabolism - starch and sucrose; Membrane protein, integral; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - pentose and glucuronate interconversions; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.4.1.17; Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Transferase

Chromosomal Location of Human Ortholog: 4q13

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane

Molecular Function: retinoic acid binding; glucuronosyltransferase activity

Biological Process: steroid metabolic process; flavonoid biosynthetic process; xenobiotic metabolic process; cellular response to hormone stimulus