Western Blot (WB)
(WB of PNPLA3 antibody (MBS544053) with:A. Rat liverB. Mouse kidneyAntibody dilution 1:500 in DiluOBufferTM (FGI-1963).Apparent MW of PNPLA3 in rat liver is 58 kDa.Apparent MW of PNPLA3 in mouse kidney is 52 kDa.Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Rabbit PNPLA3 Polyclonal Antibody | anti-PNPLA3 antibody
PNPLA3 Antibody
ELISA (EIA): 1:20,000
Immunohistochemistry (IHC): 1:50-1:100
Immunoprecipitation (IP): 1:200
Western Blot (WB): 1:500-1:750
Western Blot (WB)
(WB of PNPLA3 antibody (MBS544053) with:A. Rat liverB. Mouse kidneyAntibody dilution 1:500 in DiluOBufferTM (FGI-1963).Apparent MW of PNPLA3 in rat liver is 58 kDa.Apparent MW of PNPLA3 in mouse kidney is 52 kDa.Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Western Blot (WB)
(WB of PNPLA3 antibody (MBS544053) with:A. Rat liverB. Mouse kidneyAntibody dilution 1:500 in DiluOBufferTM (FGI-1963).Apparent MW of PNPLA3 in rat liver is 58 kDa.Apparent MW of PNPLA3 in mouse kidney is 52 kDa.Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
The PNPLA3-selective antibodies were generated against a synthetic peptide taken within amino acid region 150-200 on human Patatin-like phospholipase domain-containing protein 3.The PNPLA3 synthetic peptide was covalently modified to achiee desired antigenic properties and was conjugated to a carrier protein before used as a immunogen to raise antibodies in rabbits. The PNPLA3 antibodies are affinity purified over immobilized immunogenic peptide affinity matrix and stabilized with preservatives for long-term storage. Antigenic blocking peptide (P-PNPLA3) and western blot positive controls (PC-PNPLA3 )are available. Antibodies can be conjugated to fluorophores or secondary enzymes upon request at nominal cost.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]
Uniprot Description
PNPLA3: Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). A condition characterized by an accumulation of excess triglyceride in the liver, a condition known as hepatic steatosis (or fatty liver), which is associated with adverse metabolic consequences, including insulin resistance and dyslipidemia. In a subset of individuals, hepatic steatosis promotes an inflammatory response in the liver, referred to as steatohepatitis, which can progress to cirrhosis and liver cancer. NAFLD is the most common form of liver disease in Western countries. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Phospholipase; Lipid Metabolism - glycerophospholipid; Secondary Metabolites Metabolism - limonene and pinene degradation; EC 3.1.1.3; Lipid Metabolism - glycerolipid; Amino Acid Metabolism - phenylalanine; Transferase; Amino Acid Metabolism - tyrosine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 22q13.31
Cellular Component: endoplasmic reticulum membrane; integral to membrane; membrane
Molecular Function: acylglycerol O-acyltransferase activity; diolein transacylation activity; lipoprotein lipase activity; mono-olein transacylation activity; phospholipase A2 activity; triacylglycerol lipase activity
Biological Process: triacylglycerol biosynthetic process; triacylglycerol catabolic process
Research Articles on PNPLA3
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Product Notes
The PNPLA3 pnpla3 (Catalog #AAA544053) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PNPLA3 Antibody reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PNPLA3 can be used in a range of immunoassay formats including, but not limited to, ELISA, IP, WB. DOT Blot (DB): 1:20,000 ELISA (EIA): 1:20,000 Immunohistochemistry (IHC): 1:50-1:100 Immunoprecipitation (IP): 1:200 Western Blot (WB): 1:500-1:750. Researchers should empirically determine the suitability of the PNPLA3 pnpla3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNPLA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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