UCKL1 sirna

UCKL1 siRNA (Human)

Cat. Num. AAA8203941

• Gene Names: UCKL1; UCK1L; URKL1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UCKL1; UCKL1 siRNA (Human); URKL1; Uridine-cytidine kinase-like 1; UCKL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UCKL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 533

• Applicable Applications for UCKL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UCKL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UCKL1 sirna

siRNA to inhibit UCKL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 301129207
• NCBI GeneID: 54963
• NCBI Accession #: NP_001180308.1
• NCBI GenBank Nucleotide #: NM_001193379.1
• UniProt Accession #: Q9NWZ5; Q5JWV0; Q70AQ5; Q8N524; Q9H3Z2; B7Z8N2
• Molecular Weight: 59,466 Da
• NCBI Official Full Name: uridine-cytidine kinase-like 1 isoform 2
• NCBI Official Synonym Full Names: uridine-cytidine kinase 1-like 1
• NCBI Official Symbol: UCKL1
• NCBI Official Synonym Symbols: UCK1L; URKL1
• NCBI Protein Information: uridine-cytidine kinase-like 1
• UniProt Protein Name: Uridine-cytidine kinase-like 1
• Protein Family: Uridine-cytidine kinase
• UniProt Gene Name: UCKL1
• UniProt Synonym Gene Names: URKL1
• UniProt Entry Name: UCKL1_HUMAN

NCBI Description

The protein encoded by this gene is a uridine kinase. Uridine kinases catalyze the phosphorylation of uridine to uridine monophosphate. This protein has been shown to bind to Epstein-Barr nuclear antigen 3 as well as natural killer lytic-associated molecule. Ubiquitination of this protein is enhanced by the presence of natural killer lytic-associated molecule. In addition, protein levels decrease in the presence of natural killer lytic-associated molecule, suggesting that association with natural killer lytic-associated molecule results in ubiquitination and subsequent degradation of this protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

UCKL1: May contribute to UTP accumulation needed for blast transformation and proliferation. Belongs to the uridine kinase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; EC 2.7.1.48; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; uridine kinase activity; ATP binding

Biological Process: viral reproduction; phosphorylation