UBE2A sirna

UBE2A siRNA (Human)

Cat. Num. AAA8200705

• Gene Names: UBE2A; UBC2; HHR6A; MRXSN; RAD6A; MRXS30
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBE2A; UBE2A siRNA (Human); RAD6A; Ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UBE2A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 119

• Applicable Applications for UBE2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UBE2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBE2A sirna

siRNA to inhibit UBE2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 531990811
• NCBI GeneID: 7319
• NCBI Accession #: NP_001269090.1
• NCBI GenBank Nucleotide #: NM_001282161.1
• UniProt Accession #: P49459; Q4TTG1; Q96FX4; A6NFE9; A6NGR2; A6NMF5; B2R7R9; D3DWI1
• Molecular Weight: 8,833 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 A isoform 4
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2A
• NCBI Official Symbol: UBE2A
• NCBI Official Synonym Symbols: UBC2; HHR6A; MRXSN; RAD6A; MRXS30
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 A
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 A
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2A
• UniProt Synonym Gene Names: RAD6A; HR6A; hHR6A
• UniProt Entry Name: UBE2A_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.

Protein type: Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: XY body; cytoplasm; nuclear chromatin; cytosol; chromatin

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of peptide antigen via MHC class I; protein autoubiquitination; protein polyubiquitination; maternal process involved in pregnancy; in utero embryonic development; postreplication repair; positive regulation of cell proliferation; DNA repair; histone H2A ubiquitination; response to UV

Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type