Western Blot (WB)
(MBS645019 staining (4ug/ml) of Daudi lysate (RIPA buffer, 20ug total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.)
Goat anti-Human CD20 Polyclonal Antibody | anti-MS4A1 antibody
CD20, CT (MS4A1, B1, S7, Bp35, MS4A2, LEU-16, MGC3969, membrane-spanning 4-domains, subfamily A, member 1, B-lymphocyte cell-surface antigen B1)
Gene Names
MS4A1; CD20
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
CD20; Polyclonal Antibody; CT (MS4A1; B1; S7; Bp35; MS4A2; LEU-16; MGC3969; membrane-spanning 4-domains; subfamily A; member 1; B-lymphocyte cell-surface antigen B1); Anti -CD20; anti-MS4A1 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CD20.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-MS4A1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Immunogen
Peptide corresponding to the sequence CQDQESSPIENDSSP from the C Terminus of human CD20 (Accession #NP_068769.2; NP_690605.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MBS645019 staining (4ug/ml) of Daudi lysate (RIPA buffer, 20ug total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(MBS645019 staining (4ug/ml) of Daudi lysate (RIPA buffer, 20ug total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC)
(MBS645019 (2ug/ml) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS645019 (2ug/ml) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Related Product Information for anti-MS4A1 antibody
CD20 is a non-glycosylated phosphoprotein expressed on the surface of all mature B-cells. Membrane-spanning 4-domains, subfamily A, member 1, also known as MS4A1, is a human gene. This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. The protein has no known natural ligand and its function is unclear. It is suspected that it acts as a calcium channel in the cell membrane. CD20 is expressed on all stages of B cell development except the first and last; it is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, and B-cell chronic lymphocytic leukemia. It is also found on skin/melanoma cancer stem cells. CD20 is the target of the monoclonal antibodies rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias.
Product Categories/Family for anti-MS4A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
33,056 Da
NCBI Official Full Name
CD20
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
CD20
NCBI Protein Information
B-lymphocyte antigen CD20; membrane-spanning 4-domains subfamily A member 1
UniProt Protein Name
B-lymphocyte antigen CD20
Protein Family
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_CANFA
Uniprot Description
Function: This protein may be involved in the regulation of B-cell activation and proliferation
By similarity.
Subcellular location: Cell membrane
By similarity; Multi-pass membrane protein. Cell membrane; Lipid-anchor
By similarity.
Tissue specificity: Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells. Ref.1
Post-translational modification: Phosphorylated. Might be functionally regulated by protein kinase(s)
By similarity.
Sequence similarities: Belongs to the MS4A family.
Research Articles on MS4A1
Similar Products
Product Notes
The MS4A1 ms4a1 (Catalog #AAA645019) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The CD20, CT (MS4A1, B1, S7, Bp35, MS4A2, LEU-16, MGC3969, membrane-spanning 4-domains, subfamily A, member 1, B-lymphocyte cell-surface antigen B1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Researchers should empirically determine the suitability of the MS4A1 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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