Rat Insulin ELISA Kit | INS elisa kit
Rat Insulin ELISA Kit
Reactivity
Rat
Synonyms
Insulin; Rat Insulin ELISA Kit; Rattus norvegicus; INS; Ins; Ins-1; INS elisa kit
Reactivity
Rat
Specificity
Recombinant and natural Rat Insulin-1
Sequence Length
110
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Assay Type
Quantitative Competitive
Detection Range
0.625-40 uIU/mL
Sensitivity
0.312 uIU/mL
Intra-assay Precision
<=6.7%
Inter-assay Precision
<=9.4%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for INS elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,421 Da
NCBI Official Full Name
insulin-1 preproprotein
NCBI Official Synonym Full Names
insulin 1
NCBI Official Symbol
Ins1
NCBI Protein Information
insulin-1
UniProt Protein Name
Insulin-1
Protein Family
UniProt Gene Name
Ins1
UniProt Synonym Gene Names
Ins-1
UniProt Entry Name
INS1_RAT
NCBI Description
involved in glucose metabolism [RGD, Feb 2006]
Uniprot Description
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
