TCERG1 sirna

TCERG1 siRNA (Human)

Cat. Num. AAA8202151

• Gene Names: TCERG1; Urn1; CA150; TAF2S
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TCERG1; TCERG1 siRNA (Human); CA150; TAF2S; Transcription elongation regulator 1; TATA box-binding protein-associated factor 2S; Transcription factor CA150; TCERG1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TCERG1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1077

• Applicable Applications for TCERG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TCERG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TCERG1 sirna

siRNA to inhibit TCERG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 91208418
• NCBI GeneID: 10915
• NCBI Accession #: NP_001035095.1
• NCBI GenBank Nucleotide #: NM_001040006.1
• UniProt Accession #: O14776; Q2NKN2; Q59EA1
• Molecular Weight: 121,690 Da
• NCBI Official Full Name: transcription elongation regulator 1 isoform 2
• NCBI Official Synonym Full Names: transcription elongation regulator 1
• NCBI Official Symbol: TCERG1
• NCBI Official Synonym Symbols: Urn1; CA150; TAF2S
• NCBI Protein Information: transcription elongation regulator 1
• UniProt Protein Name: Transcription elongation regulator 1
• Protein Family: Transcription elongation regulator
• UniProt Gene Name: TCERG1
• UniProt Synonym Gene Names: CA150; TAF2S
• UniProt Entry Name: TCRG1_HUMAN

NCBI Description

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TCERG1: Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleolus; nucleus; actin cytoskeleton

Molecular Function: protein binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter