Typical Testing Data/Standard Curve (for reference only)
Monkey Clathrin heavy chain 1 (CLTC) ELISA Kit | CLTC elisa kit
Monkey Clathrin heavy chain 1 (CLTC) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CLTC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLTC antibody and an CLTC-HRP conjugate. The assay sample and buffer are incubated together with CLTC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLTC concentration since CLTC from samples and CLTC-HRP conjugate compete for the anti-CLTC antibody binding site. Since the number of sites is limited, as more sites are occupied by CLTC from the sample, fewer sites are left to bind CLTC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLTC concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
Uniprot Description
CLTC: a major protein component of the cytoplasmic face of coated vesicles and coated pits. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
Protein type: Vesicle; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q23.1
Cellular Component: clathrin coat of trans-Golgi network vesicle; protein complex; clathrin coat; focal adhesion; intracellular membrane-bound organelle; clathrin-coated vesicle; cytosol; clathrin coat of coated pit; membrane; cytoplasm; melanosome; plasma membrane; trans-Golgi network membrane; spindle; vesicle
Molecular Function: protein binding; double-stranded RNA binding; structural molecule activity; protein kinase binding; clathrin light chain binding
Biological Process: osteoblast differentiation; intracellular protein transport; receptor-mediated endocytosis; mitosis; receptor internalization; transferrin transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport
Research Articles on CLTC
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Product Notes
The Monkey CLTC cltc (Catalog #AAA7219381) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7219381 ELISA Kit recognizes Monkey CLTC. It is sometimes possible for the material contained within the vial of "Clathrin heavy chain 1 (CLTC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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