SREK1 sirna

SREK1 siRNA (Human)

Cat. Num. AAA8212383

• Gene Names: SREK1; SFRS12; SRrp86; SRrp508
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SREK1; SREK1 siRNA (Human); SFRS12; SRRP86; Splicing regulatory glutamine/lysine-rich protein 1; Serine/arginine-rich-splicing regulatory protein 86; SRrp86; Splicing factor. arginine/serine-rich 12; Splicing regulatory protein 508; SRrp508; SREK1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SREK1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 624

• Applicable Applications for SREK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SREK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SREK1 sirna

siRNA to inhibit SREK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116089325
• NCBI GeneID: 140890
• NCBI Accession #: NP_001070667.1
• NCBI GenBank Nucleotide #: NM_001077199.2
• UniProt Accession #: Q8WXA9; Q2M1J0; Q86X37; A4FTW3
• Molecular Weight: 71,649 Da
• NCBI Official Full Name: splicing regulatory glutamine/lysine-rich protein 1 isoform a
• NCBI Official Synonym Full Names: splicing regulatory glutamine/lysine-rich protein 1
• NCBI Official Symbol: SREK1
• NCBI Official Synonym Symbols: SFRS12; SRrp86; SRrp508
• NCBI Protein Information: splicing regulatory glutamine/lysine-rich protein 1
• UniProt Protein Name: Splicing regulatory glutamine/lysine-rich protein 1
• Protein Family: Splicing regulatory glutamine/lysine-rich protein
• UniProt Gene Name: SREK1
• UniProt Synonym Gene Names: SFRS12; SRRP86; SRrp86; SRrp508
• UniProt Entry Name: SREK1_HUMAN

NCBI Description

This gene encodes a member of a family of serine/arginine-rich (SR) splicing proteins containing RNA recognition motif (RRM) domains. The encoded protein interacts with other SR proteins to modulate splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

SFRS12: Participates in the regulation of alternative splicing by modulating the activity of other splice facors. Inhibits the splicing activity of SFRS1, SFRS2 and SFRS6. Augments the splicing activity of SFRS3. Belongs to the splicing factor SR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; RNA splicing

Chromosomal Location of Human Ortholog: 5q12.3

Cellular Component: nucleoplasm; spliceosome

Molecular Function: nucleotide binding

Biological Process: RNA splicing; mRNA processing