Western Blot (WB)
(Western Blot analysis of CES3 expression in transfected 293T cell line by CES3 polyclonal antibody. Lane 1: CES3 transfected lysate (62.48kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human CES3 Polyclonal Antibody | anti-CES3 antibody
CES3 (Carboxylesterase 3, Liver Carboxylesterase 31 Homolog, UNQ869/PRO1887)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of CES3 expression in transfected 293T cell line by CES3 polyclonal antibody. Lane 1: CES3 transfected lysate (62.48kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CES3 expression in transfected 293T cell line by CES3 polyclonal antibody. Lane 1: CES3 transfected lysate (62.48kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.[provided by RefSeq, Jun 2010]
Uniprot Description
Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows low catalytic efficiency for hydrolysis of CPT-11 (7-ethyl-10-[4-(1-piperidino)-1-piperidino]-carbonyloxycamptothecin), a prodrug for camptothecin used in cancer therapeutics.
Catalytic activity: A carboxylic ester + H2O = an alcohol + a carboxylate.
Subcellular location: Endoplasmic reticulum lumen
By similarity.
Tissue specificity: Expressed in liver, colon and small intestine. Ref.1 Ref.8 Ref.9
Post-translational modification: N-glycosylated. Ref.1
Sequence similarities: Belongs to the type-B carboxylesterase/lipase family.
Biophysicochemical propertiesKinetic parameters:KM=137 µM for 7-ethyl-10-[4-(1-piperidino)-1-piperidino]-carbonyloxycamptothecin Ref.1KM=460 µM for 7-ethyl-10-[4-(1-piperidino)-1-amino]-carbonyloxycamptothecin
Research Articles on CES3
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Product Notes
The CES3 ces3 (Catalog #AAA646819) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CES3 (Carboxylesterase 3, Liver Carboxylesterase 31 Homolog, UNQ869/PRO1887) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CES3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the CES3 ces3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MERAVRVESG VLVGVVCLLL ACPATATGPE VAQPEVDTTL GRVRGRQVGV KGTDRLVNVF LGIPFAQPPL GPDRFSAPHP AQPWEGVRDA STAPPMCLQD VESMNSSRFV LNGKQQIFSV SEDCLVLNVY SPAEVPAGSG RPVMVWVHGG ALITGAATSY DGSALAAYGD VVVVTVQYRL GVLGFFSTGD EHAPGNQGFL DVVAALRWVQ ENIAPFGGDL NCVTVFGGSA GGSIISGLVL SPVAAGLFHR AITQSGVITT PGIIDSHPWP LAQKIANTLA CSSSSPAEMV QCLQQKEGEE LVLSKKLKNT IYPLTVDGTV FPKSPKELLK EKPFHSVPFL MGVNNHEFSW LIPRGWGLLD TMEQMSREDM LAISTPVLTS LDVPPEMMPT VIDEYLGSNS DAQAKCQAFQ EFMGDVFINV PTVSFSRYLR DSGSPVFFYE FQHRPSSFAK IKPAWVKADH GAEGAFVFGG PFLMDESSRL EATEEEKQLS LTMMAQWTHF ARTGDPNSKA LPPWPQFNQA EQYLEINPVP RAGQKFREAW MQFWSETLPS KIQQWHQKQK NRKAQEDL. It is sometimes possible for the material contained within the vial of "CES3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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