SPATA13 sirna

SPATA13 siRNA (Human)

Cat. Num. AAA8222682

• Gene Names: SPATA13; ASEF2; ARHGEF29
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPATA13; SPATA13 siRNA (Human); Spermatogenesis-associated protein 13; APC-stimulated guanine nucleotide exchange factor 2; Asef2; SPATA13 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPATA13 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1277

• Applicable Applications for SPATA13 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPATA13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPATA13 sirna

siRNA to inhibit SPATA13 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 261337188
• NCBI GeneID: 221178
• NCBI Accession #: NP_001159743.1
• NCBI GenBank Nucleotide #: NM_001166271.2
• UniProt Accession #: Q96N96; Q5VX68; Q6ZML1; Q8N873; Q8TEK6; A2VEA9; A6NF85; B4DQB1; B4DSZ0; B4DVM8; J3KPJ7; J3KQH2
• Molecular Weight: 141,436 Da
• NCBI Official Full Name: spermatogenesis-associated protein 13 isoform 1
• NCBI Official Synonym Full Names: spermatogenesis associated 13
• NCBI Official Symbol: SPATA13
• NCBI Official Synonym Symbols: ASEF2; ARHGEF29
• NCBI Protein Information: spermatogenesis-associated protein 13
• UniProt Protein Name: Spermatogenesis-associated protein 13
• Protein Family: Spermatogenesis-associated protein
• UniProt Gene Name: SPATA13
• UniProt Synonym Gene Names: Asef2
• UniProt Entry Name: SPT13_HUMAN

Uniprot Description

SPATA13 iso6: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up- regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. Interacts (via ABR and SH3 domain) with APC. The binding of APC enhances its GEF activity by relieving it from an autoinhibitory conformation, in which the ABR and SH3 domains are associated with the C-terminal tail. Interacts (via C-terminal tail) with PPP1R9B (via C-terminus). Interacts with RAC1. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 13q12.12

Cellular Component: nucleoplasm; lamellipodium; cytoplasm; filopodium

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; Rac guanyl-nucleotide exchange factor activity

Biological Process: lamellipodium biogenesis; cell migration; filopodium formation; regulation of cell migration; positive regulation of GTPase activity