SKA3 sirna

SKA3 siRNA (Human)

Cat. Num. AAA8229078

• Gene Names: SKA3; RAMA1; C13orf3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SKA3; SKA3 siRNA (Human); C13orf3; RAMA1; Spindle and kinetochore-associated protein 3; SKA3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SKA3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 388

• Applicable Applications for SKA3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SKA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SKA3 sirna

siRNA to inhibit SKA3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 260763912
• NCBI GeneID: 221150
• NCBI Accession #: NP_001159489.1
• NCBI GenBank Nucleotide #: NM_001166017.1
• UniProt Accession #: Q8IX90; Q5VZV5; Q86WR2; Q8NBG1; Q96D22; A2A330; A2A331; B2RBY2
• Molecular Weight: 43,978 Da
• NCBI Official Full Name: spindle and kinetochore-associated protein 3 isoform 2
• NCBI Official Synonym Full Names: spindle and kinetochore associated complex subunit 3
• NCBI Official Symbol: SKA3
• NCBI Official Synonym Symbols: RAMA1; C13orf3
• NCBI Protein Information: spindle and kinetochore-associated protein 3
• UniProt Protein Name: Spindle and kinetochore-associated protein 3
• Protein Family: Spindle and kinetochore-associated protein
• UniProt Gene Name: SKA3
• UniProt Synonym Gene Names: C13orf3; RAMA1
• UniProt Entry Name: SKA3_HUMAN

NCBI Description

This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

RAMA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Belongs to the SKA3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: spindle microtubule; cytoplasm; outer kinetochore of condensed chromosome

Molecular Function: protein binding

Biological Process: mitosis; cell division; regulation of microtubule polymerization or depolymerization; chromosome segregation