Rabbit anti-Human MILK2 Polyclonal Antibody | anti-MILK2 antibody

MILK2, CT (MICALL2, MICAL-like protein 2) (Azide free) (HRP)

Cat. Num. AAA6320837

• Gene Names: MICALL2; JRAB; MICAL-L2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MILK2; Polyclonal Antibody; CT (MICALL2; MICAL-like protein 2) (Azide free) (HRP); MICALL2; MICAL-like protein 2; anti-MILK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MILK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MILK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 846-876 from the C-terminal region of human MILK2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MILK2 Antibody (C-term) western blot analysis in HL-60,CEM cell line lysates (35ug/lane).This demonstrates the MILK2 antibody detected the MILK2 protein (arrow).)

Immunohistochemistry (IHC)

(MILK2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MILK2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MILK2 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MILK2 antibody

Antibodies; Proteins

References

Kanda, I., et al. Oncogene 27(12):1687-1695(2008). Terai, T., et al. Mol. Biol. Cell 17(5):2465-2475(2006). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Terman, J.R., et al. Cell 109(7):887-900(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 33667117
• NCBI GeneID: 79778
• NCBI Accession #: NP_891554.1
• NCBI GenBank Nucleotide #: NM_182924.3
• UniProt Accession #: Q8IY33; Q7RTP4; Q7Z655; Q8TEQ4; Q9H5F9; D3YTD2
• Molecular Weight: 74,527 Da
• NCBI Official Full Name: MICAL-like protein 2
• NCBI Official Synonym Full Names: MICAL like 2
• NCBI Official Symbol: MICALL2
• NCBI Official Synonym Symbols: JRAB; MICAL-L2
• NCBI Protein Information: MICAL-like protein 2
• UniProt Protein Name: MICAL-like protein 2
• UniProt Gene Name: MICALL2
• UniProt Synonym Gene Names: JRAB; MICAL-L2

Uniprot Description

Effector of small Rab GTPases which is involved in junctional complexes assembly through the regulation of cell adhesion molecules transport to the plasma membrane and actin cytoskeleton reorganization. Regulates the endocytic recycling of occludins, claudins and E-cadherin to the plasma membrane and may thereby regulate the establishment of tight junctions and adherens junctions. In parallel, may regulate actin cytoskeleton reorganization directly through interaction with F-actin or indirectly through actinins and filamins. Most probably involved in the processes of epithelial cell differentiation, cell spreading and neurite outgrowth ().