Chicken SIRT7 Polyclonal Antibody | anti-SIRT7 antibody

SIRT7 Antibody

Cat. Num. AAA150168

• Gene Names: SIRT7; SIR2L7
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: SIRT7 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SIRT7; Polyclonal Antibody; SIRT7 Antibody; SIR2L7; NAD-dependent protein deacetylase sirtuin-7; Regulatory protein SIR2 homolog 7; sirtuin 7; anti-SIRT7 antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Purity/Purification: SIRT7 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 400

• Applicable Applications for anti-SIRT7 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: SIRT7 antibody can be used for detection of SIRT7 by Western blot at 0.5 - 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SIRT7 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human SIRT7.
• Buffer: SIRT7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SIRT7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SIRT7 in 293 cell lysate with SIRT7 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-SIRT7 antibody

SIRT7 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT7 is a nucleolar protein that positively regulates RNA polymerase I transcription and has been postulated to enable cells to sustain critical functions by inhibiting cell growth under severe stress conditions.

NCBI and Uniprot Product Information

• NCBI GI #: 7706712
• NCBI GeneID: 51547
• NCBI Accession #: NP_057622
• NCBI GenBank Nucleotide #: NM_016538.2
• UniProt Accession #: Q9NRC8; Q3MIK4; Q9NSZ6; Q9NUS6; A8K2K0; B3KSU8
• Molecular Weight: 35,851 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-7
• NCBI Official Synonym Full Names: sirtuin 7
• NCBI Official Symbol: SIRT7
• NCBI Official Synonym Symbols: SIR2L7
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-7; sirtuin type 7; SIR2-like protein 7; sir2-related protein type 7; regulatory protein SIR2 homolog 7; NAD-dependent deacetylase sirtuin-7; silent mating type information regulation 2, S.cerevisiae, homolog 7
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-7
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT7
• UniProt Synonym Gene Names: SIR2L7
• UniProt Entry Name: SIR7_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRT7: NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; EC 3.5.1.-; Deacetylase

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleolus organizer region; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; metal ion binding; chromatin binding

Biological Process: activation of transcription on exit from mitosis; negative regulation of transcription from RNA polymerase II promoter; rRNA transcription