Rabbit anti-Human RAB7B Polyclonal Antibody | anti-RAB7B antibody

RAB7B, CT (RAB7B, Ras-related protein Rab-7b) (PE)

Cat. Num. AAA6339898

• Gene Names: RAB7B; RAB7; MGC9726; MGC16212
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAB7B; Polyclonal Antibody; CT (RAB7B; Ras-related protein Rab-7b) (PE); Ras-related protein Rab-7b; anti-RAB7B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RAB7B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RAB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the C-terminal region of human RAB7B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAB7B Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RAB7B antibody detected the RAB7B protein (arrow).)

Related Product Information for anti-RAB7B antibody

Protein transport. Probably involved in vesicular traffic (By similarity).

Product Categories/Family for anti-RAB7B antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Progida, C., et al. J. Cell. Sci. 123 (PT 9), 1480-1491 (2010): Yang, M., et al. Biochem. Biophys. Res. Commun. 318(3):792-799(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 256985158
• NCBI GeneID: 338382
• NCBI Accession #: NP_796377.3
• NCBI GenBank Nucleotide #: NM_177403.4
• UniProt Accession #: Q96AH8
• Molecular Weight: 22,511 Da
• NCBI Official Full Name: ras-related protein Rab-7b
• NCBI Official Synonym Full Names: RAB7B, member RAS oncogene family
• NCBI Official Symbol: RAB7B
• NCBI Official Synonym Symbols: RAB7; MGC9726; MGC16212
• NCBI Protein Information: ras-related protein Rab-7b; Ras-related protein Rab-7
• UniProt Protein Name: Ras-related protein Rab-7b
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB7B
• UniProt Entry Name: RAB7B_HUMAN

Uniprot Description

RAB7B: Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; lysosome; late endosome; trans-Golgi network; phagocytic vesicle

Molecular Function: GTP binding

Biological Process: negative regulation of toll-like receptor 9 signaling pathway; protein transport; small GTPase mediated signal transduction; negative regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; positive regulation of megakaryocyte differentiation; activation of NF-kappaB transcription factor