SEC61A1 sirna

SEC61A1 siRNA (Human)

Cat. Num. AAA829463

• Gene Names: SEC61A1; SEC61; HSEC61; SEC61A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SEC61A1; SEC61A1 siRNA (Human); SEC61A; Protein transport protein Sec61 subunit alpha isoform 1; Sec61 alpha-1; SEC61A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SEC61A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 476

• Applicable Applications for SEC61A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SEC61A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SEC61A1 sirna

siRNA to inhibit SEC61A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7019415
• NCBI GeneID: 29927
• NCBI Accession #: NP_037468.1
• NCBI GenBank Nucleotide #: NM_013336.3
• UniProt Accession #: P61619; P38378; P57726; Q5JPF8; Q8N0Z4; Q8N3U3; Q8NC71; Q9BU16; Q9Y2R3
• Molecular Weight: 38,849 Da
• NCBI Official Full Name: protein transport protein Sec61 subunit alpha isoform 1
• NCBI Official Synonym Full Names: Sec61 translocon alpha 1 subunit
• NCBI Official Symbol: SEC61A1
• NCBI Official Synonym Symbols: SEC61; HSEC61; SEC61A
• NCBI Protein Information: protein transport protein Sec61 subunit alpha isoform 1
• UniProt Protein Name: Protein transport protein Sec61 subunit alpha isoform 1
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC61A1
• UniProt Synonym Gene Names: SEC61A; Sec61 alpha-1
• UniProt Entry Name: S61A1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008]

Uniprot Description

SEC61A1: Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane- bound ribosomes, either directly or through adapter proteins. Belongs to the SecY/SEC61-alpha family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: membrane; rough endoplasmic reticulum; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; ribosome binding

Biological Process: endoplasmic reticulum organization and biogenesis; protein targeting to ER; SRP-dependent cotranslational protein targeting to membrane; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; posttranslational protein targeting to membrane; cell growth