SEC23B sirna

SEC23B siRNA (Human)

Cat. Num. AAA8200583

• Gene Names: SEC23B; CDAII; CDAN2; CDA-II; HEMPAS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SEC23B; SEC23B siRNA (Human); Protein transport protein Sec23B; SEC23-related protein B; SEC23B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SEC23B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 767

• Applicable Applications for SEC23B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SEC23B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SEC23B sirna

siRNA to inhibit SEC23B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 289629265
• NCBI GeneID: 10483
• NCBI Accession #: NP_001166216.1
• NCBI GenBank Nucleotide #: NM_001172745.1
• UniProt Accession #: Q15437; Q503A9; Q5W183; Q9BS15; Q9BSI2; Q9H1D7; D3DW33
• Molecular Weight: 86,479 Da
• NCBI Official Full Name: protein transport protein Sec23B isoform 1
• NCBI Official Synonym Full Names: Sec23 homolog B, COPII coat complex component
• NCBI Official Symbol: SEC23B
• NCBI Official Synonym Symbols: CDAII; CDAN2; CDA-II; HEMPAS
• NCBI Protein Information: protein transport protein Sec23B
• UniProt Protein Name: Protein transport protein Sec23B
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC23B
• UniProt Entry Name: SC23B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

SEC23B: Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and Sar1. Belongs to the SEC23/SEC24 family. SEC23 subfamily.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; intracellular membrane-bound organelle; membrane; COPII vesicle coat; perinuclear region of cytoplasm; endomembrane system

Molecular Function: zinc ion binding

Biological Process: vesicle-mediated transport; intracellular protein transport; ER to Golgi vesicle-mediated transport

Disease: Anemia, Congenital Dyserythropoietic, Type Ii