Human protein transport protein Sec23B ELISA Kit | Sec23b elisa kit

Human protein transport protein Sec23B ELISA Kit

Cat. Num. AAA762355

• Gene Names: SEC23B; CDAII; CDAN2; CDA-II; HEMPAS
• Reactivity: Human
• Synonyms: protein transport protein Sec23B; Human protein transport protein Sec23B ELISA Kit; Sec23b/SEC23-related protein B/SEC23B; Sec23b elisa kit

• Reactivity: Human
• Sequence Length: 749

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Sec23b elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human Sec23b antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Sec23b antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Sec23b amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Sec23b can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 289629267
• NCBI GeneID: 10483
• NCBI Accession #: NP_001166217.1
• NCBI GenBank Nucleotide #: NM_001172746.1
• Molecular Weight: 86,479 Da
• NCBI Official Full Name: protein transport protein Sec23B isoform 2
• NCBI Official Synonym Full Names: Sec23 homolog B, COPII coat complex component
• NCBI Official Symbol: SEC23B
• NCBI Official Synonym Symbols: CDAII; CDAN2; CDA-II; HEMPAS
• NCBI Protein Information: protein transport protein Sec23B
• UniProt Protein Name: Protein transport protein Sec23B
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC23B
• UniProt Entry Name: SC23B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

SEC23B: Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and Sar1. Belongs to the SEC23/SEC24 family. SEC23 subfamily.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: COPII vesicle coat; endomembrane system; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; Golgi membrane; intracellular membrane-bound organelle; membrane; perinuclear region of cytoplasm

Molecular Function: protein binding; zinc ion binding

Biological Process: ER to Golgi vesicle-mediated transport; intracellular protein transport; vesicle-mediated transport

Disease: Anemia, Congenital Dyserythropoietic, Type Ii