RPA4 sirna

RPA4 siRNA (Human)

Cat. Num. AAA8225466

• Gene Names: RPA4; HSU24186
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RPA4; RPA4 siRNA (Human); Replication protein A 30 kDa subunit; RP-A p30; Replication factor A protein 4; RF-A protein 4; RPA4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RPA4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 261

• Applicable Applications for RPA4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RPA4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RPA4 sirna

siRNA to inhibit RPA4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9558731
• NCBI GeneID: 29935
• NCBI Accession #: NP_037479.1
• NCBI GenBank Nucleotide #: NM_013347.4
• UniProt Accession #: Q13156; Q3SY03
• Molecular Weight: 28,868 Da
• NCBI Official Full Name: replication protein A 30 kDa subunit
• NCBI Official Synonym Full Names: replication protein A4, 30kDa
• NCBI Official Symbol: RPA4
• NCBI Official Synonym Symbols: HSU24186
• NCBI Protein Information: replication protein A 30 kDa subunit
• UniProt Protein Name: Replication protein A 30 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA4
• UniProt Synonym Gene Names: RP-A p30; RF-A protein 4
• UniProt Entry Name: RFA4_HUMAN

NCBI Description

This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010]

Uniprot Description

RPA4: functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. Interacts with RPA1 and RPA3. Localizes to DNA repair foci after DNA damage.

Chromosomal Location of Human Ortholog: Xq21.33

Cellular Component: nucleoplasm; DNA replication factor A complex; nucleus

Molecular Function: single-stranded DNA binding

Biological Process: DNA replication initiation; nucleotide-excision repair; DNA damage checkpoint; DNA replication during S phase; mitotic cell cycle; DNA recombination; G1/S transition of mitotic cell cycle