Rabbit anti-Human Connexin 31 Polyclonal Antibody | anti-Cx31 antibody

Connexin 31, CT, C241 (Gap Junction beta-3 Protein, Connexin-31, Cx31, GJB3) (FITC)

Cat. Num. AAA6470049

• Gene Names: GJB3; EKV; CX31; DFNA2; EKVP1; DFNA2B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Connexin 31; Polyclonal Antibody; CT; C241 (Gap Junction beta-3 Protein; Connexin-31; Cx31; GJB3) (FITC); anti-Cx31 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJB3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 270

• Applicable Applications for anti-Cx31 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa234-264 from the C-terminal region of human GJB3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hGJB3-C241 in WiDr cell line lysates (35ug/lane). GJB3 (arrow) was detected using the purified Pab.(2ug/ml))

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Cx31 antibody

Antibodies; Connexin; Junction Proteins

References

1. Mhatre, A.N., et al., Clin. Genet. 63(2):154-159 (2003). 2. Diestel, S., et al., Biochem. Biophys. Res. Commun. 296(3):721-728 (2002). 3. Di, W.L., et al., Hum. Mol. Genet. 11(17):2005-2014 (2002). 4. Gottfried, I., et al., Hum. Mol. Genet. 11(11):1311-1316 (2002). 5. Wenzel, K., et al., Biochem. Biophys. Res. Commun. 248(3):910-915 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 13128960
• NCBI GeneID: 2707
• NCBI Accession #: NP_076872
• NCBI GenBank Nucleotide #: NP_076872.1
• UniProt Accession #: O75712
• Molecular Weight: 30.818
• NCBI Official Full Name: gap junction beta-3 protein
• NCBI Official Synonym Full Names: gap junction protein beta 3
• NCBI Official Symbol: GJB3
• NCBI Official Synonym Symbols: EKV; CX31; DFNA2; EKVP1; DFNA2B
• NCBI Protein Information: gap junction beta-3 protein
• UniProt Protein Name: Gap junction beta-3 protein
• UniProt Gene Name: GJB3
• UniProt Synonym Gene Names: CX31; Cx31
• UniProt Entry Name: CXB3_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

GJB3: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJB3 are a cause of erythrokeratodermia variabilis (EKV). EKV is a genodermatosis characterized by the appearance of two independent skin lesions: transient figurate erythematous patches and hyperkeratosis that is usually localized but occasionally occurs in its generalized form. Clinical presentation varies significantly within a family and from one family to another. Palmoplantar keratoderma is present in around 50% of cases. Defects in GJB3 are the cause of deafness autosomal dominant type 2B (DFNA2B). DFNA2 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: connexon complex; cytoplasm; gap junction; integral to membrane

Molecular Function: gap junction channel activity

Biological Process: skin development; sensory perception of sound; in utero embryonic development; cell communication; transmembrane transport; placenta development

Disease: Deafness, Autosomal Recessive 1a; Deafness, Autosomal Dominant 2b; Erythrokeratodermia Variabilis Et Progressiva