RFFL sirna
RFFL siRNA (Human)
Gene Names
RFFL; CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN
Reactivity
Human
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
RFFL; RFFL siRNA (Human); RNF189; RNF34L; E3 ubiquitin-protein ligase rififylin; Caspase regulator CARP2; Caspases-8 and -10-associated RING finger protein 2; CARP-2; FYVE-RING finger protein Sakura; Fring; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING finger protein 34-like; RFFL sirna
Host
Synthetic
Reactivity
Human
Specificity
RFFL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
363
Applicable Applications for RFFL sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RFFL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for RFFL sirna
siRNA to inhibit RFFL expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,633 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase rififylin
NCBI Official Synonym Full Names
ring finger and FYVE-like domain containing E3 ubiquitin protein ligase
NCBI Official Symbol
RFFL
NCBI Official Synonym Symbols
CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN
NCBI Protein Information
E3 ubiquitin-protein ligase rififylin
UniProt Protein Name
E3 ubiquitin-protein ligase rififylin
Protein Family
UniProt Gene Name
RFFL
UniProt Synonym Gene Names
CARP-2; Fring
UniProt Entry Name
RFFL_HUMAN
Uniprot Description
RFFL: Has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Vesicle
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: Golgi membrane; membrane; lysosome; recycling endosome membrane; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; endosome membrane; cytosol
Molecular Function: protein binding; protease binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; protein kinase binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; intracellular protein transport; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of TOR signaling pathway
Research Articles on RFFL
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Product Notes
The RFFL rffl (Catalog #AAA8228702) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The RFFL siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RFFL can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the RFFL rffl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFFL, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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