Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7045766 at dilution 1:100)
Rabbit anti-Human RFFL Polyclonal Antibody | anti-RFFL antibody
RFFL Antibody
Gene Names
RFFL; CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
RFFL; Polyclonal Antibody; RFFL Antibody; E3 ubiquitin-protein ligase rififylinCurated; Caspase regulator CARP2; Caspases-8 and -10-associated RING finger protein 2; CARP-2; FYVE-RING finger protein Sakura; Fring; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING finger protein 34-like; anti-RFFL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
363
Applicable Applications for anti-RFFL antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: IHC: 1:20-1:200
Species
Human
Immunogen
Recombinant human E3 ubiquitin-protein ligase rififylin protein (N-190AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7045766 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7045766 at dilution 1:100)
Related Product Information for anti-RFFL antibody
E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,633 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase rififylin
NCBI Official Synonym Full Names
ring finger and FYVE like domain containing E3 ubiquitin protein ligase
NCBI Official Symbol
RFFL
NCBI Official Synonym Symbols
CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN
NCBI Protein Information
E3 ubiquitin-protein ligase rififylin
UniProt Protein Name
E3 ubiquitin-protein ligase rififylin
Protein Family
UniProt Gene Name
RFFL
UniProt Synonym Gene Names
CARP-2; Fring
UniProt Entry Name
RFFL_HUMAN
Uniprot Description
RFFL: Has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin ligase; Vesicle; Ligase; Ubiquitin conjugating system; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: cytoplasm; endosome membrane; Golgi membrane; lysosome; membrane; nucleoplasm; plasma membrane
Molecular Function: p53 binding; protease binding; protein binding; protein kinase binding; ubiquitin protein ligase binding
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of TOR signaling pathway; ubiquitin-dependent protein catabolic process
Research Articles on RFFL
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Product Notes
The RFFL rffl (Catalog #AAA7045766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RFFL Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RFFL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). Recommended dilution: IHC: 1:20-1:200. Researchers should empirically determine the suitability of the RFFL rffl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFFL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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