Mouse Sirtuin 6 (SIRT6) RTU ELISA Kit | SIRT6 rtu elisa kit

Mouse Sirtuin 6 (SIRT6) ELISA Kit

Cat. Num. AAA4502512

• Gene Names: Sirt6; Sir2l6; AI043036; 2810449N18Rik
• Reactivity: Mouse
• Synonyms: Sirtuin 6 (SIRT6); Mouse Sirtuin 6 (SIRT6) ELISA Kit; SIR2L6; Silent Mating Type Information Regulation 2 Homolog 6; Mono-ADP-Ribosyltransferase Sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; SIRT6 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 334

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.063ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIRT6 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRT6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SIRT6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SIRT6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SIRT6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31712018
• NCBI GeneID: 50721
• NCBI Accession #: NP_853617.1
• NCBI GenBank Nucleotide #: NM_181586.3
• UniProt Accession #: P59941
• Molecular Weight: 36,920 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-6 isoform 1
• NCBI Official Synonym Full Names: sirtuin 6
• NCBI Official Symbol: Sirt6
• NCBI Official Synonym Symbols: Sir2l6; AI043036; 2810449N18Rik
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-6
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: Sirt6
• UniProt Synonym Gene Names: Sir2l6

Uniprot Description

NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD+ in the absence of acetylated substrate (). Acts as a corepressor of the transcription factor Hif1a to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. Regulates the production of TNF protein. Has a role in the regulation of life span in male mice, but not in female mice.