PVR sirna

PVR siRNA (Human)

Cat. Num. AAA8234583

• Gene Names: PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PVR; PVR siRNA (Human); PVS; Poliovirus receptor; Nectin-like protein 5; NECL-5; CD antigen CD155; PVR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PVR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 372

• Applicable Applications for PVR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PVR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PVR sirna

siRNA to inhibit PVR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209413728
• NCBI GeneID: 5817
• NCBI Accession #: NP_001129240.1
• NCBI GenBank Nucleotide #: NM_001135768.2
• UniProt Accession #: P15151; P15152; Q15267; Q15268; Q96BJ1; B4DTS9
• Molecular Weight: 42,864 Da
• NCBI Official Full Name: poliovirus receptor isoform beta
• NCBI Official Synonym Full Names: poliovirus receptor
• NCBI Official Symbol: PVR
• NCBI Official Synonym Symbols: PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• NCBI Protein Information: poliovirus receptor
• UniProt Protein Name: Poliovirus receptor
• Protein Family: Poliovirus receptor
• UniProt Gene Name: PVR
• UniProt Synonym Gene Names: PVS; NECL-5
• UniProt Entry Name: PVR_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

PVR: Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. Belongs to the nectin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; cell surface; focal adhesion; cytoplasm; integral to membrane; plasma membrane

Molecular Function: viral receptor activity; protein binding; cell adhesion molecule binding; receptor activity

Biological Process: intercellular junction assembly and maintenance; regulation of immune response; entry of virus into host cell; cell migration; cell-cell adhesion; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; susceptibility to natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity