Typical Testing Data/Standard Curve (for reference only)
Rat Phenol Sulfotransferase (PST) ELISA Kit | PST elisa kit
Rat Phenol Sulfotransferase (PST) ELISA Kit
Gene Names
Sult1a1; Stm; Stp1; ASTIV; Mx-ST; PST-1; St1a1; Sult1a3
Reactivity
Rat
Synonyms
Phenol Sulfotransferase (PST); Rat Phenol Sulfotransferase (PST) ELISA Kit; SULT1A1; HAST1/HAST2; ST1A3; STP1; TSPST1; Sulfotransferase 1A1; Aryl sulfotransferase 1; Phenol sulfotransferase 1; Thermostable phenol sulfotransferase; PST elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of PST. No significant cross-reactivity or interference between PST and analogues was observed.
Sequence Length
291
Samples
Rat tissue homogenates, cell lysates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.2-2000pg/mL
Sensitivity
< 12.4pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PST were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PST were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PST elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PST in rat tissue homogenates, cell lysates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,906 Da
NCBI Official Full Name
sulfotransferase 1A1
NCBI Official Synonym Full Names
sulfotransferase family 1A member 1
NCBI Official Symbol
Sult1a1
NCBI Official Synonym Symbols
Stm; Stp1; ASTIV; Mx-ST; PST-1; St1a1; Sult1a3
NCBI Protein Information
sulfotransferase 1A1
UniProt Protein Name
Sulfotransferase 1A1
UniProt Gene Name
Sult1a1
UniProt Synonym Gene Names
St1a1; ST1A1; ASTIV; Mx-ST
Uniprot Description
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.
Research Articles on PST
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Product Notes
The Rat PST sult1a1 (Catalog #AAA452148) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA452148 ELISA Kit recognizes Rat PST. It is sometimes possible for the material contained within the vial of "Phenol Sulfotransferase (PST), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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