Rabbit anti-Human C4orf49 Polyclonal Antibody | anti-MGARP antibody

C4orf49 Antibody (C-term)

Cat. Num. AAA9201967

• Gene Names: MGARP; OSAP; HUMMR; CESP-1; C4orf49
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A column, followed by peptide affinity purification.
• Synonyms: C4orf49; Polyclonal Antibody; C4orf49 Antibody (C-term); Protein MGARP; Corneal endothelium-specific protein 1; CESP-1; Hypoxia up-regulated mitochondrial movement regulator protein; Mitochondria-localized glutamic acid-rich protein; Ovary-specific acidic protein; MGARP; CESP1; HUMMR; OSAP; anti-MGARP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This C4orf49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the C-terminal region of human C4orf49.
• Purity/Purification: Protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 176-204
• Sequence Length: 240

• Applicable Applications for anti-MGARP antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-paraffin (IHC-P)
• Application Notes: WB~~1:1000; IHC-P~~1:10~50
• Function: Plays a role in the trafficking of mitochondria along microtubules. Regulates the kinesin-mediated axonal transport of mitochondria to nerve terminals along microtubules during hypoxia. Participates in the translocation of TRAK2/GRIF1 from the cytoplasm to the mitochondrion. Also plays a role in steroidogenesis through maintenance of mitochondrial abundance and morphology (By similarity).
• Cellular Location: Mitochondrion. Mitochondrion outer membrane {ECO:0000250|UniProtKB:Q8VI64}; Single-pass type IV membrane protein {ECO:0000250|UniProtKB:Q8VI64}; Cytoplasmic side {ECO:0000250|UniProtKB:Q8VI64}. Note=Colocalizes with RHOT1, RHOT2, TRAK1 and TRAK2 at the mitochondrion. {ECO:0000250|UniProtKB:Q8VI64}
• Tissue Location: Expressed in the brain, adrenal gland and corneal endothelium (CE). Expressed in steroid-producing cells of the ovary and testis (at protein level). Expressed in steroid-producing cells of the ovary and testis. Weakly expressed in placenta. Expressed in corneal endothelial cells.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze/thaw cycles.

Western Blot (WB)

(C4orf49 Antibody (C-term) (MBS9201967) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C4orf49 antibody detected the C4orf49 protein (arrow).)

Immunohistochemistry (IHC)

(C4orf49 Antibody (C-term) (MBS9201967) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of C4orf49 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MGARP antibody

The function of this protein remains unknown.

Product Categories/Family for anti-MGARP antibody

Cardiovascular; Metabolism; Signal transduction

References

Matsumoto, T., et al. Endocrinology 150(7):3353-3359(2009)
Li, Y., et al. J. Cell Biol. 185(6):1065-1081(2009)
Kinouchi, R., et al. Invest. Ophthalmol. Vis. Sci. 47(4):1397-1403(2006)
Sakai, R., et al. Invest. Ophthalmol. Vis. Sci. 43(6):1749-1756(2002)

NCBI and Uniprot Product Information

• NCBI GI #: 117168279
• NCBI GeneID: 84709
• NCBI Accession #: NP_116012.2
• NCBI GenBank Nucleotide #: NM_032623.3
• UniProt Accession #: Q8TDB4; Q9BZC3
• Molecular Weight: 25390
• NCBI Official Full Name: protein MGARP
• NCBI Official Synonym Full Names: mitochondria-localized glutamic acid-rich protein
• NCBI Official Symbol: MGARP
• NCBI Official Synonym Symbols: OSAP; HUMMR; CESP-1; C4orf49
• NCBI Protein Information: protein MGARP
• UniProt Protein Name: Protein MGARP
• Protein Family: Protein
• UniProt Gene Name: MGARP
• UniProt Synonym Gene Names: C4orf49; CESP1; HUMMR; OSAP; CESP-1
• UniProt Entry Name: HUMMR_HUMAN

Uniprot Description

OSAP: Plays a role in the trafficking of mitochondria along microtubules. Regulates the kinesin-mediated axonal transport of mitochondria to nerve terminals along microtubules during hypoxia. Participates in the translocation of TRAK2/GRIF1 from the cytoplasm to the mitochondrion. Also plays a role in steroidogenesis through maintenance of mitochondrial abundance and morphology.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q31.1

Cellular Component: mitochondrion; integral to mitochondrial outer membrane

Molecular Function: protein binding

Biological Process: axon transport of mitochondrion; anterograde axon cargo transport; retrograde axon cargo transport; protein targeting to mitochondrion