Rabbit EAR2 Polyclonal Antibody | anti-EAR2 antibody

EAR2 (S40) Polyclonal Antibody

Cat. Num. AAA3001883

• Gene Names: NR2F6; EAR2; EAR-2; ERBAL2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EAR2; Polyclonal Antibody; EAR2 (S40) Polyclonal Antibody; Probable glutamyl-tRNA synthetase; mitochondrial; Glutamate--tRNA ligase; GluRS; EARS2; KIAA1970; anti-EAR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EAR2 (S40) polyclonal antibody detects endogenous levels of EAR2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 404

• Applicable Applications for anti-EAR2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 21-70 of Human EAR2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of EAR2  (S40)  pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of EAR2 (S40) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-EAR2 antibody

EAR2 (v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2) protein binds a cis enhancer element (TGACCT motif) upstream of the rennin gene and represses renin gene transcription. EAR2 also contributes to gonadotropin-dependent derepression of LHR promoter activity in granulose cells. EAR2 mRNA is abundant in the liver, and EAR2 protein localizes to the nucleus of As4.1 cells.

NCBI and Uniprot Product Information

• NCBI GI #: 20070199
• NCBI GeneID: 2063
• NCBI Accession #: NP_005225.2
• NCBI GenBank Nucleotide #: NP_005225.2
• UniProt Accession #: P10588
• Molecular Weight: ~ 42kDa
• NCBI Official Full Name: nuclear receptor subfamily 2 group F member 6
• NCBI Official Synonym Full Names: nuclear receptor subfamily 2 group F member 6
• NCBI Official Symbol: NR2F6
• NCBI Official Synonym Symbols: EAR2; EAR-2; ERBAL2
• NCBI Protein Information: nuclear receptor subfamily 2 group F member 6
• UniProt Protein Name: Nuclear receptor subfamily 2 group F member 6
• Protein Family: Eosinophil cationic protein
• UniProt Gene Name: NR2F6
• UniProt Synonym Gene Names: EAR2; ERBAL2; EAR-2
• UniProt Entry Name: NR2F6_HUMAN

Uniprot Description

EAR-2: Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type- specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC). Belongs to the nuclear hormone receptor family. NR2 subfamily.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; neuron development; gene expression; steroid hormone mediated signaling; negative regulation of transcription from RNA polymerase II promoter; signal transduction; detection of temperature stimulus involved in sensory perception of pain; entrainment of circadian clock by photoperiod