PRDM6 sirna

PRDM6 siRNA (Human)

Cat. Num. AAA8222065

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRDM6; PRDM6 siRNA (Human); PFM3; Putative histone-lysine N-methyltransferase PRDM6; PR domain zinc finger protein 6; PR domain-containing protein 6; PRDM6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRDM6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 595

• Applicable Applications for PRDM6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRDM6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRDM6 sirna

siRNA to inhibit PRDM6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 210031233
• NCBI GeneID: 93166
• NCBI Accession #: NP_001129711.1
• NCBI GenBank Nucleotide #: NM_001136239.1
• UniProt Accession #: Q9NQX0; Q9NQW9; B5MCJ4
• Molecular Weight: 46,635 Da
• NCBI Official Full Name: putative histone-lysine N-methyltransferase PRDM6
• NCBI Official Synonym Full Names: PR domain containing 6
• NCBI Official Symbol: PRDM6
• NCBI Protein Information: putative histone-lysine N-methyltransferase PRDM6
• UniProt Protein Name: Putative histone-lysine N-methyltransferase PRDM6
• Protein Family: Putative histone-lysine N-methyltransferase
• UniProt Gene Name: PRDM6
• UniProt Synonym Gene Names: PFM3
• UniProt Entry Name: PRDM6_HUMAN

Uniprot Description

PRDM6: Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; EC 2.1.1.43; Methyltransferase, protein lysine; C2H2-type zinc finger protein; Methyltransferase

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; nucleic acid binding; metal ion binding; histone-lysine N-methyltransferase activity

Biological Process: transcription, DNA-dependent; neurogenesis; negative regulation of smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent