Human MST1 ELISA Kit | STK4 elisa kit

Human MST1 ELISA Kit

Cat. Num. AAA824743

• Gene Names: STK4; KRS2; MST1; YSK3; TIIAC
• Reactivity: Human
• Applications: ELISA
• Synonyms: MST1; Human MST1 ELISA Kit; KRS2; Serine/threonine-protein kinase 4; Mammalian STE20-like protein kinase 1; MST-1; STE20-like kinase MST1; Serine/threonine-protein kinase Krs-2; STK4 elisa kit

• Reactivity: Human
• Specificity: The Human IL-15 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-15 proteins within the range of 7.8 pg/ml-500 pg/ml.
• Sequence Length: 487

• Applicable Applications for STK4 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma
• Sensitivity: 4 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STK4 elisa kit

Principle of the Assay: MBS Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-15 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Interleukin (IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.

NCBI and Uniprot Product Information

• NCBI GI #: 5454096
• NCBI GeneID: 6789
• NCBI Accession #: NP_006273.1
• NCBI GenBank Nucleotide #: NM_006282.2
• UniProt Accession #: Q13043; Q15802; Q4G156; Q5H982; Q6PD60; Q9BR32; Q9NTZ4; B2RCR8
• Molecular Weight: 52,335 Da
• NCBI Official Full Name: serine/threonine-protein kinase 4
• NCBI Official Synonym Full Names: serine/threonine kinase 4
• NCBI Official Symbol: STK4
• NCBI Official Synonym Symbols: KRS2; MST1; YSK3; TIIAC
• NCBI Protein Information: serine/threonine-protein kinase 4; MST-1; STE20-like kinase MST1; mammalian sterile 20-like 1; kinase responsive to stress 2; mammalian STE20-like protein kinase 1; serine/threonine-protein kinase Krs-2; dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2))
• UniProt Protein Name: Serine/threonine-protein kinase 4
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK4
• UniProt Synonym Gene Names: KRS2; MST1; MST-1; MST1/N; MST1/C
• UniProt Entry Name: STK4_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]

Uniprot Description

MST1: a protein kinase of the STE20 family. Proteolytically activated by caspase during apoptosis. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, STE; Autophagy; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MST subfamily

Chromosomal Location of Human Ortholog: 20q11.2-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein dimerization activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; identical protein binding; protein binding; protein homodimerization activity; magnesium ion binding; transcription factor binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: central nervous system development; positive regulation of protein binding; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; cell morphogenesis; negative regulation of organ growth; signal transduction; protein amino acid phosphorylation; patterning of blood vessels; keratinocyte differentiation; negative regulation of cell proliferation; peptidyl-serine phosphorylation; positive regulation of fat cell differentiation; neural tube formation

Disease: T-cell Immunodeficiency, Recurrent Infections, And Autoimmunity With Or Without Cardiac Malformations