PNLIPRP2 sirna

PNLIPRP2 siRNA (Human)

Cat. Num. AAA8222619

• Gene Names: PNLIPRP2; PLRP2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PNLIPRP2; PNLIPRP2 siRNA (Human); PLRP2; Pancreatic lipase-related protein 2; PL-RP2; Galactolipase; PNLIPRP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PNLIPRP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 470

• Applicable Applications for PNLIPRP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PNLIPRP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PNLIPRP2 sirna

siRNA to inhibit PNLIPRP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 106507261
• NCBI GeneID: 5408
• NCBI Accession #: NP_005387.2
• NCBI GenBank Nucleotide #: NM_005396.4
• UniProt Accession #: P54317; Q6IB55; A8K627
• Molecular Weight: 51,947 Da
• NCBI Official Full Name: pancreatic lipase-related protein 2
• NCBI Official Synonym Full Names: pancreatic lipase-related protein 2
• NCBI Official Symbol: PNLIPRP2
• NCBI Official Synonym Symbols: PLRP2
• NCBI Protein Information: pancreatic lipase-related protein 2
• UniProt Protein Name: Pancreatic lipase-related protein 2
• Protein Family: Pancreatic lipase-related protein
• UniProt Gene Name: PNLIPRP2
• UniProt Synonym Gene Names: PLRP2; PL-RP2
• UniProt Entry Name: LIPR2_HUMAN

NCBI Description

This gene encodes a lipase that hydrolyzes galactolipids, the main components of plant membrane lipids. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the non-coding allele. [provided by RefSeq, Aug 2015]

Uniprot Description

PNLIPRP2: Lipase with broad substrate specificity. Can hydrolyze both phospholipids and galactolipids. Acts preferentially on monoglycerides, phospholipids and galactolipids. Contributes to milk fat hydrolysis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted; EC 3.1.1.3; Lipase; Secreted, signal peptide; EC 3.1.1.26; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: extracellular space; extracellular region

Molecular Function: triacylglycerol lipase activity; galactolipase activity; acylglycerol lipase activity; calcium ion binding; phospholipase activity

Biological Process: triacylglycerol metabolic process; phospholipid catabolic process; galactolipid catabolic process; lipid digestion