PIGZ sirna

PIGZ siRNA (Human)

Cat. Num. AAA8217064

• Gene Names: PIGZ; SMP3; PIG-Z; GPI-MT-IV
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIGZ; PIGZ siRNA (Human); SMP3; GPI mannosyltransferase 4; GPI mannosyltransferase IV; GPI-MT-IV; Phosphatidylinositol-glycan biosynthesis class Z protein; PIG-Z; SMP3 homolog; hSMP3; PIGZ sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PIGZ siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 579

• Applicable Applications for PIGZ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PIGZ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIGZ sirna

siRNA to inhibit PIGZ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30581137
• NCBI GeneID: 80235
• NCBI Accession #: NP_079439.2
• NCBI GenBank Nucleotide #: NM_025163.3
• UniProt Accession #: Q86VD9; Q9H9G6
• Molecular Weight: 63,471 Da
• NCBI Official Full Name: GPI mannosyltransferase 4
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis, class Z
• NCBI Official Symbol: PIGZ
• NCBI Official Synonym Symbols: SMP3; PIG-Z; GPI-MT-IV
• NCBI Protein Information: GPI mannosyltransferase 4
• UniProt Protein Name: GPI mannosyltransferase 4
• UniProt Gene Name: PIGZ
• UniProt Synonym Gene Names: SMP3; GPI-MT-IV; PIG-Z; hSMP3
• UniProt Entry Name: PIGZ_HUMAN

NCBI Description

The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq, Jul 2008]

Uniprot Description

PIGZ: Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-GPIs during GPI precursor assembly. The presence of a fourth mannose in GPI is facultative and only scarcely detected, suggesting that it only exists in some tissues. Belongs to the glycosyltransferase 22 family. PIGZ subfamily.

Protein type: Transferase; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; EC 2.4.1.-; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: mannosyltransferase activity; alpha-1,2-mannosyltransferase activity

Biological Process: GPI anchor biosynthetic process