Cadherin 17/CDH17 Recombinant Protein | CDH17 recombinant protein

Cadherin 17/CDH17 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120797

• Gene Names: CDH17; HPT1; CDH16; HPT-1
• Purity: >88% as determined by SDS-PAGE
• Synonyms: Cadherin 17/CDH17; Cadherin 17/CDH17 Protein; Human; Recombinant (His Tag); Human Cadherin-17/LI-cadherin/CDH17 Protein (His Tag); CDH16 Protein; HPT-1 Protein; HPT1 Protein; cadherin 17; LI cadherin (liver-intestine); CDH17 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >88% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Met787

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CDH17 (Q12864-1) extracellular domain (Met 1-Met 787) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDH17 recombinant protein

Background: Cadherin-17 or LI-cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Cadherin-17/LI-cadherin is a cadherin-like protein consisting of an extracellular region, 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing of the encoding gene results in multiple transcript variants. Cadherin-17/LI-cadherin preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin-17 may thus contribute to the sorting of heterogeneous cell types and have a role in the morphological organization of liver and intestine. It's also involved in intestinal peptide transport. Experiments have reported the association between Cadherin-17/LI-cadherin and gastric cancer. Cadherin-17/LI-cadherin expression was detected in 63/94 of gastric adenocarcinomas in addition to intestinal metaplasia. The expression of Cadherin-17 tended to be associated with intestinal type carcinoma, and carcinomas with Cadherin-17 expression was significantly more frequent in advanced stage cases than in early stage. Cadherin-17 is also a useful immunohistochemical marker for test of adenocarcinomas of the digestive system.

Product Categories/Family for CDH17 recombinant protein

Canonical Wnt Pathway

References

Liu LX, et al. (2009) Targeting cadherin-17 inactivates Wnt signaling and inhibits tumor growth in liver carcinoma. Hepatology. 50(5): 1453-63.Ito R, et al. (2005) Clinicopathological significant and prognostic influence of cadherin-17 expression in gastric cancer. Virchows Arch. 447(4): 717-22.Horsfield J, et al. (2002) Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development. Mech Dev. 115(1-2): 15-26.

NCBI and Uniprot Product Information

• NCBI GI #: 221316595
• NCBI GeneID: 1015
• NCBI Accession #: NP_001138135.1
• NCBI GenBank Nucleotide #: NM_001144663.1
• UniProt Accession #: Q12864; Q15336; Q2M2E0
• Molecular Weight: 92,219 Da
• NCBI Official Full Name: cadherin-17
• NCBI Official Synonym Full Names: cadherin 17, LI cadherin (liver-intestine)
• NCBI Official Symbol: CDH17
• NCBI Official Synonym Symbols: HPT1; CDH16; HPT-1
• NCBI Protein Information: cadherin-17; LI cadherin; LI-cadherin; cadherin-16; HPT-1 cadherin; liver-intestine cadherin; human peptide transporter 1; intestinal peptide-associated transporter HPT-1; human intestinal peptide-associated transporter HPT-1
• UniProt Protein Name: Cadherin-17
• Protein Family: Cadherin
• UniProt Gene Name: CDH17
• UniProt Synonym Gene Names: LI-cadherin
• UniProt Entry Name: CAD17_HUMAN

NCBI Description

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

Uniprot Description

CDH17: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: transporter activity; calcium ion binding; proton-dependent oligopeptide secondary active transmembrane transporter activity

Biological Process: intercellular junction assembly and maintenance; transport; calcium-dependent cell-cell adhesion; oligopeptide transport; homophilic cell adhesion; cell adhesion