PIEZO1 sirna

PIEZO1 siRNA (Human)

Cat. Num. AAA8233905

• Gene Names: PIEZO1; DHS; Mib; FAM38A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIEZO1; PIEZO1 siRNA (Human); FAM38A; KIAA0233; Piezo-type mechanosensitive ion channel component 1; Membrane protein induced by beta-amyloid treatment; Mib; Protein FAM38A; PIEZO1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PIEZO1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2521

• Applicable Applications for PIEZO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PIEZO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIEZO1 sirna

siRNA to inhibit PIEZO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 257196142
• NCBI GeneID: 9780
• NCBI Accession #: NP_001136336.2
• NCBI GenBank Nucleotide #: NM_001142864.3
• UniProt Accession #: Q92508; Q0KKZ9; A6NHT9; A7E2B7
• Molecular Weight: 286,790 Da
• NCBI Official Full Name: piezo-type mechanosensitive ion channel component 1
• NCBI Official Synonym Full Names: piezo-type mechanosensitive ion channel component 1
• NCBI Official Symbol: PIEZO1
• NCBI Official Synonym Symbols: DHS; Mib; FAM38A
• NCBI Protein Information: piezo-type mechanosensitive ion channel component 1
• UniProt Protein Name: Piezo-type mechanosensitive ion channel component 1
• Protein Family: Piezo-type mechanosensitive ion channel component
• UniProt Gene Name: PIEZO1
• UniProt Synonym Gene Names: FAM38A; KIAA0233; Mib
• UniProt Entry Name: PIEZ1_HUMAN

NCBI Description

The protein encoded by this gene is a mechanically-activated ion channel that links mechanical forces to biological signals. The encoded protein contains 36 transmembrane domains and functions as a homotetramer. Defects in this gene have been associated with dehydrated hereditary stomatocytosis. [provided by RefSeq, Jul 2015]

Uniprot Description

PIEZO1: Component of mechanosensitive channel required for the mechanosensitive currents. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. Belongs to the PIEZO family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; endoplasmic reticulum; integral to membrane; plasma membrane

Molecular Function: mechanically-gated ion channel activity; cation channel activity

Biological Process: regulation of membrane potential; detection of mechanical stimulus; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of integrin activation; cation transport

Disease: Dehydrated Hereditary Stomatocytosis With Or Without Pseudohyperkalemia And/or Perinatal Edema