Typical Testing Data/Standard Curve (for reference only)
Rabbit VLA ELISA Kit | VLA elisa kit
Rabbit VLA (Very Late Appearing Antigen) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VLA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VLA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VLA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VLA. You can calculate the concentration of VLA in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor for arginine vasopressin, a neurohypophyseal hormone involved in diuresis inhibition, smooth muscle contraction, liver glycogenolysis stimulation and regulation of adrenocorticotropic hormone release from the pituitary. This receptor represents one of three G protein-coupled arginine vasopressin receptors which functions through a phosphotidylinositol-calcium second messenger system in vascular and hepatic tissues. [provided by RefSeq, Jul 2008]
Uniprot Description
AVPR1A: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl- inositol-calcium second messenger system. Has been involved in social behaviors, including affiliation and attachment. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Cellular Component: cytoplasmic vesicle membrane; integral to plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle
Molecular Function: peptide hormone binding; V1A vasopressin receptor binding; vasopressin receptor activity
Biological Process: positive regulation of cellular pH reduction; positive regulation of glutamate secretion; cellular response to water deprivation; positive regulation of systemic arterial blood pressure; positive regulation of glycogen catabolic process; response to organic substance; elevation of cytosolic calcium ion concentration; positive regulation of cell proliferation; penile erection; response to corticosterone stimulus; positive regulation of prostaglandin biosynthetic process; grooming behavior; response to inorganic substance; maternal behavior; calcium-mediated signaling; myotube differentiation; positive regulation of heart rate; social behavior; positive regulation of cell growth; cellular response to hormone stimulus; negative regulation of female receptivity; G-protein coupled receptor protein signaling pathway; telencephalon development; negative regulation of transmission of nerve impulse; regulation of systemic arterial blood pressure by vasopressin; regulation of adrenocorticotropin hormone secretion; positive regulation of vasoconstriction; sperm ejaculation; positive regulation of blood pressure
Research Articles on VLA
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Product Notes
The Rabbit VLA avpr1a (Catalog #AAA2507274) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2507274 ELISA Kit recognizes Rabbit VLA. It is sometimes possible for the material contained within the vial of "VLA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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