Immunofluorescence (IF)
(Huvecs transfected with Piezo1-GFP, Staining with mouse anti-human Piezo1 antibody (5 ug/ml, MBS435119). No Permeablized. Recommended dilution: 10 ug/mlIF-Anti-PIEZO1 Antibody MBS435119)
Mouse anti-Homo Sapiens PIEZO1 Monoclonal Antibody | anti-PIEZO1 antibody
PIEZO1 Mouse mAb
Immunofluorescence: 1:100-200
FACS: 1:100-200
For Western Blot (WB), incubate membrane with diluted antibdoy in 5% w/v nonfat dry milk, 1x TBS, 0.05% Tween-20 at 4°C with gentle shaking overnight.
Immunofluorescence (IF)
(Huvecs transfected with Piezo1-GFP, Staining with mouse anti-human Piezo1 antibody (5 ug/ml, MBS435119). No Permeablized. Recommended dilution: 10 ug/mlIF-Anti-PIEZO1 Antibody MBS435119)
Immunofluorescence (IF)
(Huvecs transfected with Piezo1-GFP, Staining with mouse anti-human Piezo1 antibody (5 ug/ml, MBS435119). No Permeablized. Recommended dilution: 10 ug/mlIF-Anti-PIEZO1 Antibody MBS435119)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling PIEZO1 with MBS435119 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling PIEZO1 with MBS435119 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a mechanically-activated ion channel that links mechanical forces to biological signals. The encoded protein contains 36 transmembrane domains and functions as a homotetramer. Defects in this gene have been associated with dehydrated hereditary stomatocytosis. [provided by RefSeq, Jul 2015]
Uniprot Description
Pore-forming subunit of a mechanosensitive non-specific cation channel (PubMed:23479567, PubMed:23695678). Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling (PubMed:20016066). In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation (PubMed:25119035). Plays a key role in blood vessel formation and vascular structure in both development and adult physiology ().
Research Articles on PIEZO1
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Product Notes
The PIEZO1 piezo1 (Catalog #AAA435119) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PIEZO1 Mouse mAb reacts with Homo Sapiens and may cross-react with other species as described in the data sheet. AAA Biotech's PIEZO1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Immunoprecipitation: 10 tests Immunofluorescence: 1:100-200 FACS: 1:100-200 For Western Blot (WB), incubate membrane with diluted antibdoy in 5% w/v nonfat dry milk, 1x TBS, 0.05% Tween-20 at 4°C with gentle shaking overnight. Researchers should empirically determine the suitability of the PIEZO1 piezo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIEZO1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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