MUC7 sirna

MUC7 siRNA (Human)

Cat. Num. AAA8228492

• Gene Names: MUC7; MG2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MUC7; MUC7 siRNA (Human); MG2; Mucin-7; MUC-7; Apo-MG2; Salivary mucin-7; MUC7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MUC7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 377

• Applicable Applications for MUC7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MUC7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MUC7 sirna

siRNA to inhibit MUC7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 222418647
• NCBI GeneID: 4589
• NCBI Accession #: NP_001138478.1
• NCBI GenBank Nucleotide #: NM_001145006.1
• UniProt Accession #: Q8TAX7; Q9UCD7; Q9UCD8
• Molecular Weight: 39,159 Da
• NCBI Official Full Name: mucin-7
• NCBI Official Synonym Full Names: mucin 7, secreted
• NCBI Official Symbol: MUC7
• NCBI Official Synonym Symbols: MG2
• NCBI Protein Information: mucin-7
• UniProt Protein Name: Mucin-7
• Protein Family: Mucin
• UniProt Gene Name: MUC7
• UniProt Synonym Gene Names: MG2; MUC-7
• UniProt Entry Name: MUC7_HUMAN

NCBI Description

This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. This antimicrobial protein has antibacterial and antifungal activity. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

MUC7: May function in a protective capacity by promoting the clearance of bacteria in the oral cavity and aiding in mastication, speech, and swallowing. Binds P.aeruginosa pili. Genetic variations in MUC7 are associated with susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: Golgi lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification

Disease: Asthma, Susceptibility To