Goat TAF7L Polyclonal Antibody | anti-TAF7L antibody

Goat anti-TAF7L Antibody

Cat. Num. AAA421860

• Gene Names: TAF7L; CT40; TAF2Q
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TAF7L; Polyclonal Antibody; Goat anti-TAF7L Antibody; TAF7-like RNA polymerase II; TATA box binding protein (TBP)-associated factor; 50kDa; FLJ23157; TAF2Q; dJ738A13.1; OTTHUMP00000023675; 50 kD; TATA box binding protein (T; TAF7L antibody; 50kDa antibody; FLJ23157 antibody; TAF2Q antibody; dJ738A13.1 antibody; OTTHUMP00000023675 antibody; 50 kD antibody; RNA polymerase II; Q antibody; TATA box binding protein-associated factor; TBP-associated factor; anti-TAF7L antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: NHFQSVLEQLELQEK
• Sequence Length: 462

• Applicable Applications for anti-TAF7L antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx 40kDa band observed in Human Testis lysates (calculated MW of 52.6kDa according to NP_079161.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-NHFQSVLEQLELQEK, from the internal region (near C Terminus) of the protein sequence according to NP_079161.2.
• Epitope: Internal region (near C Terminus)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 51477718
• NCBI GeneID: 54457
• NCBI Accession #: NP_079161.2
• NCBI GenBank Nucleotide #: NM_024885.2
• Molecular Weight: 34,726 Da
• NCBI Official Full Name: TATA box binding protein-associated factor, RNA polymerase II, Q
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 7 like
• NCBI Official Symbol: TAF7L
• NCBI Official Synonym Symbols: CT40; TAF2Q
• NCBI Protein Information: transcription initiation factor TFIID subunit 7-like
• UniProt Protein Name: Transcription initiation factor TFIID subunit 7-like
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF7L
• UniProt Synonym Gene Names: TAF2Q; CT40
• UniProt Entry Name: TAF7L_HUMAN

NCBI Description

This gene is similar to a mouse gene that encodes a TATA box binding protein-associated factor, and shows testis-specific expression. The encoded protein could be a spermatogenesis-specific component of the DNA-binding general transcription factor complex TFIID. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

TAF7L: Probably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis. Belongs to the TAF7 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); DNA-binding

Chromosomal Location of Human Ortholog: Xq22.1

Cellular Component: cytoplasm; transcription factor TFIID complex

Molecular Function: histone acetyltransferase binding; transcription coactivator activity; transcription factor binding

Biological Process: cell differentiation; multicellular organismal development; regulation of transcription from RNA polymerase II promoter; spermatogenesis; transcriptional preinitiation complex assembly