MAP4K2 sirna

MAP4K2 siRNA (Human)

Cat. Num. AAA8230211

• Gene Names: MAP4K2; GCK; BL44; RAB8IP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAP4K2; MAP4K2 siRNA (Human); GCK; RAB8IP; Mitogen-activated protein kinase kinase kinase kinase 2; B lymphocyte serine/threonine-protein kinase; Germinal center kinase; GC kinase; MAPK/ERK kinase kinase kinase 2; MEK kinase kinase 2; MEKKK 2; Rab8-interacting protein; MAP4K2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAP4K2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 812

• Applicable Applications for MAP4K2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAP4K2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAP4K2 sirna

siRNA to inhibit MAP4K2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 808175990
• NCBI GeneID: 5871
• NCBI Accession #: NP_001294919.1
• NCBI GenBank Nucleotide #: NM_001307990.1
• UniProt Accession #: Q12851; Q86VU3
• Molecular Weight: 90,809 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 2 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 2
• NCBI Official Symbol: MAP4K2
• NCBI Official Synonym Symbols: GCK; BL44; RAB8IP
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 2
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 2
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K2
• UniProt Synonym Gene Names: GCK; RAB8IP; GC kinase; MEK kinase kinase 2; MEKKK 2
• UniProt Entry Name: M4K2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

GCK: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. Interacts with TRAF2, TRAF6, MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with RAB8A. Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined. The tumor necrosis factor (TNF), as well as endotoxins and proinflammatory stimuli such as polyinosine- polycytidine (poly(IC)), lipopolysaccharides (LPS), peptidoglycan (PGN), flagellin, or lipid A activate MAP4K2 by promoting its autophosphorylation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: Protein kinase, STE; Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: Golgi membrane; basolateral plasma membrane; cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; mitogen-activated protein kinase kinase kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: vesicle targeting; innate immune response; JNK cascade; positive regulation of JNK cascade; immune response; protein amino acid phosphorylation; activation of JNK activity