Rabbit MAP4K2 Polyclonal Antibody | anti-MAP4K2 antibody

MAP4K2 Rabbit Polyclonal

Cat. Num. AAA7601214

• Gene Names: MAP4K2; GCK; BL44; RAB8IP
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: MAP4K2; Polyclonal Antibody; MAP4K2 Rabbit Polyclonal; anti-MAP4K2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 812

• Applicable Applications for anti-MAP4K2 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Mitogen-activated protein kinase kinase kinase kinase 2
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(mouse brain tissue were subjected to SDS PAGE followed by western blot with MBS7601214 (MAP4K2 antibody) at dilution of 1:300)

NCBI and Uniprot Product Information

• NCBI GI #: 28839779
• NCBI GeneID: 5871
• Molecular Weight: 90,809 Da
• NCBI Official Full Name: MAP4K2 protein
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 2
• NCBI Official Symbol: MAP4K2
• NCBI Official Synonym Symbols: GCK; BL44; RAB8IP
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 2
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 2
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K2
• UniProt Synonym Gene Names: GCK; RAB8IP; GC kinase; MEK kinase kinase 2; MEKKK 2
• UniProt Entry Name: M4K2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

GCK: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. Interacts with TRAF2, TRAF6, MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with RAB8A. Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined. The tumor necrosis factor (TNF), as well as endotoxins and proinflammatory stimuli such as polyinosine- polycytidine (poly(IC)), lipopolysaccharides (LPS), peptidoglycan (PGN), flagellin, or lipid A activate MAP4K2 by promoting its autophosphorylation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: EC 2.7.11.1; Protein kinase, STE; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: basolateral plasma membrane; Golgi membrane

Molecular Function: ATP binding; MAP kinase kinase kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of JNK activity; activation of MAPKKK activity; immune response; innate immune response; JNK cascade; positive regulation of JNK cascade; protein amino acid phosphorylation; vesicle targeting