Western Blot (WB)
(Anti-OGFR rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:73 kDa)
Rabbit anti-Human OGFR Polyclonal Antibody | anti-OGFR antibody
Anti-OGFR Antibody, Rabbit Polyclonal
IP: 5-10uL/mg of lysate
Western Blot (WB)
(Anti-OGFR rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:73 kDa)
Western Blot (WB)
(Anti-OGFR rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:73 kDa)
Immunoprecipitation (IP)
(OGFR was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-OGFR rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-OGFR rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 73 kDa Observed band size :90 kDa)
Immunoprecipitation (IP)
(OGFR was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-OGFR rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-OGFR rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 73 kDa Observed band size :90 kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a receptor for opioid growth factor (OGF), also known as [Met(5)]-enkephalin. OGF is a negative regulator of cell proliferation and tissue organization in a variety of processes. The encoded unbound receptor for OGF has been localized to the outer nuclear envelope, where it binds OGF and is translocated into the nucleus. The coding sequence of this gene contains a polymorphic region of 60 nt tandem imperfect repeat units. Several transcripts containing between zero and eight repeat units have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
OGFR: Receptor for opioid growth factor (OGF), also known as Met-enkephalin. Seems to be involved in growth regulation. Belongs to the opioid growth factor receptor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.
Chromosomal Location of Human Ortholog: 20q13.3
Cellular Component: membrane; cytoplasm; nucleus
Molecular Function: opioid receptor activity
Biological Process: regulation of cell growth
Research Articles on OGFR
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Product Notes
The OGFR ogfr (Catalog #AAA8103678) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-OGFR Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OGFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 5-10uL/mg of lysate. Researchers should empirically determine the suitability of the OGFR ogfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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