KLF13 sirna

KLF13 siRNA (Human)

Cat. Num. AAA8227524

• Gene Names: KLF13; BTEB3; FKLF2; NSLP1; RFLAT1; RFLAT-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KLF13; KLF13 siRNA (Human); BTEB3; NSLP1; Krueppel-like factor 13; Basic transcription element-binding protein 3; BTE-binding protein 3; Novel Sp1-like zinc finger transcription factor 1; RANTES factor of late activated T-lymphocytes 1; RFLAT-1; Transcription factor BTEB3; Transcription factor NSLP1; KLF13 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KLF13 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 288

• Applicable Applications for KLF13 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KLF13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KLF13 sirna

siRNA to inhibit KLF13 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 698320875
• NCBI GeneID: 51621
• NCBI Accession #: NP_001289390.1
• NCBI GenBank Nucleotide #: NM_001302461.1
• UniProt Accession #: Q9Y2Y9; Q9Y356
• Molecular Weight: 31,180 Da
• NCBI Official Full Name: Krueppel-like factor 13 isoform 2
• NCBI Official Synonym Full Names: Kruppel-like factor 13
• NCBI Official Symbol: KLF13
• NCBI Official Synonym Symbols: BTEB3; FKLF2; NSLP1; RFLAT1; RFLAT-1
• NCBI Protein Information: Krueppel-like factor 13
• UniProt Protein Name: Krueppel-like factor 13
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF13
• UniProt Synonym Gene Names: BTEB3; NSLP1; BTE-binding protein 3; RFLAT-1
• UniProt Entry Name: KLF13_HUMAN

NCBI Description

KLF13 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes (Scohy et al., 2000 [PubMed 11087666]).[supplied by OMIM, Mar 2008]

Uniprot Description

KLF13: Represses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES expression in T-cells. Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 15q12

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter

Disease: Chromosome 15q13.3 Deletion Syndrome