Goat OAS2 Polyclonal Antibody | anti-OAS2 antibody

Goat anti-OAS2 (aa357-371) Antibody

Cat. Num. AAA423395

• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: OAS2; Polyclonal Antibody; Goat anti-OAS2 (aa357-371) Antibody; Goat Anti-OAS2 Antibody; 2'-5'-oligoadenylate synthetase 2; 69/71kDa; (2'-5')oligo(A) synthetase 2; 2'-5'-oligoadenylate synthase 2; 2-5A synthase 2; p69 OAS / p71 OAS; OAS2 antibody; 69/71kDa antibody; (2'-5')oligo(A) synthetase 2 antibody; 2'-5'-oligoadenylate synthase 2 antibody; 2-5A synthase 2 antibody; p69 OAS / p71 OAS antibody; OAS2 (aa357-371); anti-OAS2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QPNKCFLEQIDSAVN
• Sequence Length: 719

• Applicable Applications for anti-OAS2 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 70kDa band observed in lysates of cell lines Daudi, Jurkat and K562 (calculated MW of 78.8kDa according to NP_002526.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lin et al, J Immunol.2009 Dec 15;183(12): 8035-43. PMID: 19923450). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence QPNKCFLEQIDSAVN, from the internal region of the protein sequence according to NP_058197.2; NP_002526.2.
• Epitope: Internal region
• Note: This antibody is expected to recognize reported isoform 1 (NP_058197.2) and isoform 2 (NP_002526.2).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Daudi (A), Jurkat (B) and K562 (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 74229019
• NCBI GeneID: 4939
• NCBI Accession #: NP_058197.2; NP_002526.2
• NCBI GenBank Nucleotide #: NM_016817.2
• Molecular Weight: 19,517 Da
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase 2 isoform 1
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase 2
• NCBI Official Symbol: OAS2
• NCBI Protein Information: 2'-5'-oligoadenylate synthase 2
• UniProt Protein Name: 2'-5'-oligoadenylate synthase 2
• Protein Family: 2'-5'-oligoadenylate synthase
• UniProt Gene Name: OAS2
• UniProt Synonym Gene Names: (2-5')oligo(A) synthase 2; 2-5A synthase 2; p69OAS / p71OAS
• UniProt Entry Name: OAS2_HUMAN

NCBI Description

This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

OAS2: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Belongs to the 2-5A synthase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.7.7.84

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; intracellular membrane-bound organelle; membrane; mitochondrion; nucleus; perinuclear region of cytoplasm

Molecular Function: 2'-5'-oligoadenylate synthetase activity; ATP binding; double-stranded RNA binding; metal ion binding; protein binding; zinc ion binding

Biological Process: defense response to virus; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; protein amino acid glycosylation; protein myristoylation; purine nucleotide biosynthetic process; response to virus; RNA catabolic process