GSTO1 sirna

GSTO1 siRNA (Human)

Cat. Num. AAA8205425

• Gene Names: GSTO1; P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GSTO1; GSTO1 siRNA (Human); GSTTLP28; Glutathione S-transferase omega-1; GSTO-1; Glutathione S-transferase omega 1-1; GSTO 1-1; Glutathione-dependent dehydroascorbate reductase; Monomethylarsonic acid reductase; MMA(V) reductase; S-(Phenacyl)glutathione reductase; SPG-R; GSTO1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GSTO1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 208

• Applicable Applications for GSTO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GSTO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GSTO1 sirna

siRNA to inhibit GSTO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 300360532
• NCBI GeneID: 9446
• NCBI Accession #: NP_001177931.1
• NCBI GenBank Nucleotide #: NM_001191002.1
• UniProt Accession #: P78417; Q5TA03; Q7Z3T2; D3DRA3; F5H7H0
• Molecular Weight: 24,796 Da
• NCBI Official Full Name: glutathione S-transferase omega-1 isoform 2
• NCBI Official Synonym Full Names: glutathione S-transferase omega 1
• NCBI Official Symbol: GSTO1
• NCBI Official Synonym Symbols: P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21
• NCBI Protein Information: glutathione S-transferase omega-1
• UniProt Protein Name: Glutathione S-transferase omega-1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTO1
• UniProt Synonym Gene Names: GSTTLP28; GSTO-1; GSTO 1-1; MMA(V) reductase; SPG-R
• UniProt Entry Name: GSTO1_HUMAN

NCBI Description

The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

GSTO1: Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. Belongs to the GST superfamily. Omega family.

Protein type: EC 1.20.4.2; EC 1.8.5.1; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10q25.1

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; glutathione transferase activity; glutathione dehydrogenase (ascorbate) activity; methylarsonate reductase activity; oxidoreductase activity

Biological Process: positive regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; xenobiotic metabolic process; L-ascorbic acid metabolic process; xenobiotic catabolic process