GNRH2 sirna

GNRH2 siRNA (Human)

Cat. Num. AAA8220772

• Gene Names: GNRH2; GnRH-II; LH-RHII
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GNRH2; GNRH2 siRNA (Human); Progonadoliberin-2; Progonadoliberin II; GNRH2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GNRH2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 120

• Applicable Applications for GNRH2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GNRH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GNRH2 sirna

siRNA to inhibit GNRH2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4504057
• NCBI GeneID: 2797
• NCBI Accession #: NP_001492.1
• NCBI GenBank Nucleotide #: NM_001501.1
• UniProt Accession #: O43555; Q14C68; Q14C69; Q9BYN9; Q9BYP0
• Molecular Weight: 12,233 Da
• NCBI Official Full Name: progonadoliberin-2 isoform a preproprotein
• NCBI Official Synonym Full Names: gonadotropin-releasing hormone 2
• NCBI Official Symbol: GNRH2
• NCBI Official Synonym Symbols: GnRH-II; LH-RHII
• NCBI Protein Information: progonadoliberin-2
• UniProt Protein Name: Progonadoliberin-2
• Protein Family: Progonadoliberin
• UniProt Gene Name: GNRH2
• UniProt Synonym Gene Names: GnRH II; LH-RH II
• UniProt Entry Name: GON2_HUMAN

NCBI Description

This gene encodes a secreted peptide hormone and member of the gonadotropin-releasing hormone (GnRH) family of proteins. The encoded protein regulates reproductive function by stimulating the production and release of the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The encoded protein may inhibit endometrial, ovarian, prostate, and breast cancer cell proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

GNRH2: Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. Belongs to the GnRH family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: multicellular organismal development; signal transduction